Impact of pubertal onset on region‐specific Esr2 expression
| Autor: | Elli P. Sellinger, Janice M. Juraska, Carly M. Drzewiecki |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Gene isoform
medicine.medical_specialty Endocrinology Diabetes and Metabolism Prefrontal Cortex Striatum Biology Article Cellular and Molecular Neuroscience Endocrinology Internal medicine Cortex (anatomy) medicine Animals Estrogen Receptor beta Rats Long-Evans Sexual Maturation Receptor Prefrontal cortex Messenger RNA Endocrine and Autonomic Systems Motor Cortex Corpus Striatum Rats medicine.anatomical_structure Organ Specificity Female Motor cortex Puberty onset |
| Zdroj: | J Neuroendocrinol |
| ISSN: | 1365-2826 0953-8194 |
| DOI: | 10.1111/jne.13029 |
| Popis: | In female rats, pubertal onset is associated with maturation of the medial prefrontal cortex (mPFC) and mPFC-mediated behaviors. These behavioral and anatomical changes are likely due to effects of estrogens at the nuclear estrogen receptor beta (ERβ), which is expressed at higher levels than the estrogen receptor alpha (ERα) isoform in the adult mPFC. Researchers have previously quantified ERβ protein and Esr2 RNA in rodents during early postnatal development and adulthood, but an adolescent-specific trajectory of this receptor in the mPFC has not been documented. Given that Esr2 expression can fluctuate in the presence or absence of estrogens, puberty and the subsequent rise in gonadal hormones could influence levels of ERβ in the adolescent brain. To further explore this, we used RNAscope to quantify the amount of Esr2 mRNA in pre-pubertal adolescent, recently post-pubertal adolescent, and adult female rats. We show here that Esr2 expression decreases significantly in the mPFC, striatum and motor cortex between pre-pubertal adolescence and adulthood. In the mPFC, this decrease occurs rapidly at pubertal onset, with no significant decrease in Esr2 levels between the recently post-pubertal and adult cohort. In contrast, the striatum and motor cortex had no significant differences in the amount of Esr2 mRNA between pre- and post-pubertal females. Insofar as the amount of Esr2 expression is proportional to functional ERβ, these results suggest ERβ decreases in a region-specific pattern in response to pubertal onset and highlight a role for this receptor in the maturational events that occur in the female rat mPFC at puberty. |
| Databáze: | OpenAIRE |
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