Induction and removal of DNA damage in blood leukocytes of patients with type 2 diabetes mellitus undergoing hemodialysis
| Autor: | Pamela Brambilla Bagatini, Roberta Passos Palazzo, Sharbel Weidner Maluf, Manoela Tressoldi Rodrigues, Cynthia Hernandes Costa |
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| Rok vydání: | 2008 |
| Předmět: |
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medicine.medical_specialty DNA Repair DNA repair DNA damage Health Toxicology and Mutagenesis medicine.medical_treatment Type 2 diabetes Lesion Renal Dialysis Internal medicine Leukocytes Genetics medicine Humans Aged chemistry.chemical_classification Reactive oxygen species business.industry Type 2 Diabetes Mellitus Middle Aged medicine.disease Comet assay Oxidative Stress Endocrinology Diabetes Mellitus Type 2 chemistry Female Comet Assay Hemodialysis medicine.symptom business DNA Damage |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 657:111-115 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2008.08.004 |
| Popis: | Type 2 diabetes mellitus (T2DM) is associated with a high production of reactive oxygen species, which may cause oxidative DNA damage. High levels of genomic damage have been associated with renal failure and hemodialysis. However, no information is available in the literature concerning the levels of DNA damage in T2DM individuals who are dependent on hemodialysis. This study used the comet assay to assess the levels of DNA damage before, immediately after and 48 h after the hemodialysis session in 25 patients with T2DM and in a group of 20 healthy individuals, selected according to mean age, sex and smoking habit. Our results showed increased levels of DNA damage in hemodialysis-dependent T2DM individuals (12.36 ± 8.04) when compared with healthy individuals (7.35 ± 7.41) (p = 0.014). Damage levels increased immediately after the hemodialysis session (19.76 ± 12.40) (p = 0.04), which suggests a possible action of pro-oxidative factors related to the therapy, with a genotoxic effect on cells. Results obtained 48 h after hemodialysis (6.44 ± 5.99) evidenced damage removal (p = 0.001), which may be suggestive of DNA repair. |
| Databáze: | OpenAIRE |
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