Microneme protein 8 - a new essential invasion factor in Toxoplasma gondii
| Autor: | Stephan Hegge, Dominique Soldati-Favre, Angelika Herm-Götz, Friedrich Frischknecht, Markus Meissner, Manuel Rauch, Henning Kessler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Protozoan Proteins
Fluorescent Antibody Technique Cell Line Microneme Organelle parasitic diseases Parasite hosting Animals Humans Secretion ddc:616 Protozoan Proteins/genetics/metabolism/physiology biology Rhoptry Models Genetic Toxoplasma gondii Cell Biology biology.organism_classification Cell biology Toxoplasma/genetics/growth & development/metabolism Cell Adhesion Molecules/genetics/metabolism/physiology Cytosol Cell Adhesion Molecules Toxoplasma Function (biology) |
| Zdroj: | Journal of Cell Science, Vol. 121, No Pt 7 (2008) pp. 947-56 |
| ISSN: | 0021-9533 |
| Popis: | Apicomplexan parasites rely on sequential secretion of specialised secretory organelles for the invasion of the host cell. First, micronemes release their content upon contact with the host cell. Second, rhoptries are discharged, leading to the formation of a tight interaction (moving junction) with the host cell, through which the parasite invades. The functional characterisation of several micronemal proteins in Toxoplasma gondii suggests the occurrence of a stepwise process. Here, we show that the micronemal protein MIC8 of T. gondii is essential for the parasite to invade the host cell. When MIC8 is not present, a block in invasion is caused by the incapability of the parasite to form a moving junction with the host cell. We furthermore demonstrate that the cytosolic domain is crucial for the function of MIC8 and can not be functionally complemented by any other micronemal protein characterised so far, suggesting that MIC8 represents a novel, functionally distinct invasion factor in this apicomplexan parasite. |
| Databáze: | OpenAIRE |
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