Spread of ESC-, carbapenem- and colistin-resistant Escherichia coli clones and plasmids within and between food workers in Lebanon
| Autor: | Antoine Drapeau, Jean-Yves Madec, Monzer Hamze, Marwan Osman, Hiba Al-Mir, Marisa Haenni |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
clone (Java method) Adult Carbapenem Single-nucleotide polymorphism Microbial Sensitivity Tests Biology medicine.disease_cause Genome Microbiology Plasmid medicine Escherichia coli Humans Pharmacology (medical) Lebanon Escherichia coli Infections Pharmacology Colistin Escherichia coli Proteins Anti-Bacterial Agents Clone Cells Infectious Diseases Carriage Carbapenems medicine.drug Plasmids |
| Zdroj: | The Journal of antimicrobial chemotherapy. 76(12) |
| ISSN: | 1460-2091 |
| Popis: | Objectives Knowledge on the dynamic of MDR Escherichia coli in the human community is still limited, especially in low- and middle-income countries. Our goal was to decipher the dynamics of E. coli lineages and plasmids resistant to ESC, carbapenem and colistin within and between food workers in Lebanon using genomic-based approaches. Methods Eighty-four healthy adults working in three bakeries were sampled twice at a 6 monthly interval. E. coli resistant to ESC (ESC-E), carbapenem (CP-E) and colistin (CO-E) were collected on selective plates. Non-duplicate isolates were whole-genome sequenced using the Illumina technology and plasmid transmission was assessed by long-read sequencing. Data were analysed using bioinformatics tools and SNP-based phylogeny. Results ESC-E carriage rate reached 34.5% (t0) and 52.9% (t6), and 15 workers were positive at both t0 and t6. Carbapenem resistance (blaOXA-181, blaOXA-204, blaNDM-5) was found in five workers at t0 and two at t6, while colistin resistance (mcr-1.1) was found in five workers at t0 and one at t6. Forty-seven different STs were identified, of which three STs were predominant (ST131, n = 9; ST10, n = 5; ST69, n = 5). One worker presented the same ESC-E clone at t0 and t6. Twelve different events of clonal transmission among individuals were exemplified while plasmid transmission was only shown once. Conclusions Our study revealed a high carriage rate of MDR E. coli (60.7%) and the emergence of CP and colistin resistance in the Lebanese community. Incidental and long-term ESC-E carriage was observed in 41.7% and 17.9% of the workers, respectively. The high clonal diversity suggests an important dynamic of acquisition and loss of MDR E. coli and limited plasmid spread. |
| Databáze: | OpenAIRE |
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