The estimation of phenylalanine in serum by paper chromatography at elevated temperatures
| Autor: | S.K. Wadman, Josephina M. van de Heuvel |
|---|---|
| Rok vydání: | 1960 |
| Předmět: |
chemistry.chemical_classification
Chromatography Fever Ion exchange Chromatography Paper Chemistry Phenylalanine Biochemistry (medical) Clinical Biochemistry Temperature Phenylpyruvic acid General Medicine Normal values Biochemistry Amino acid Excretion Paper chromatography chemistry.chemical_compound Serum phenylalanine level Humans Regression Analysis |
| Zdroj: | Clinica Chimica Acta. 5:824-827 |
| ISSN: | 0009-8981 |
| Popis: | A paper Chromatographic method for the analysis of phenylalanine in serum is described. The amino acids, including phenylalanine, are isolated from the deproteinised serum by means of a cation exchanger. The phenylalanine is then separated by paper chromatography at elevated temperature and estimated colorimetrically. Normal values are given. The excretion of phenylalanine and phenylpyruvic acid is correlated with the serum phenylalanine level. The method is specific and the reproducibility is good. Its application in a case of phenylketonuria is reported. |
| Databáze: | OpenAIRE |
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