A preliminary comparison of flow cytometry and tube agglutination assays in detecting red blood cell-associated C3d
| Autor: | Jo L. Procter, Joyce M. Njoroge, Jeffery L. Miller, Richard W. Childs, David F. Stroncek |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Anemia Hemolytic Erythrocytes Lymphocyte Immunoglobulins Sensitivity and Specificity Flow cytometry Coombs test medicine Humans Tube (fluid conveyance) Diagnostic Errors medicine.diagnostic_test biology Reproducibility of Results Hemagglutination Tests Hematology Flow Cytometry Haemolysis Molecular biology Agglutination (biology) Red blood cell medicine.anatomical_structure Complement C3d Complement C3b biology.protein Antibody |
| Zdroj: | Transfusion Medicine. 13:35-42 |
| ISSN: | 1365-3148 0958-7578 |
| DOI: | 10.1046/j.1365-3148.2003.00415.x |
| Popis: | Summary. This study compared flow cytometric analysis with tube agglutination assays for the detection of red blood cell (RBC)-associated complement and immunoglobulins (Igs). RBCs from 20 patients with reactive tube direct antiglobulin tests (DATs) were evaluated by flow cytometry with anti-C3d, anti-IgG, anti-IgM and anti-IgA. Serial samples were also tested from a patient at risk of passenger lymphocyte haemolysis. Results of flow cytometry and tube assays for anti-IgG were as follows: 12 of 20 samples reactive in both; six of 20 nonreactive in both; two of 20 discordant with a reactive tube and a nonreactive flow cytometry assay. Anti-C3d results showed nine of 20 reactive in both and 11 of 20 discordant with a nonreactive tube and a reactive flow cytometry assay. In the IgM flow cytometry assay, three samples were reactive with anti-IgM. Samples from a group A woman who was transplanted with stem cells from a group B donor showed that on days 3 through 6 post-transplant, the flow cytometry assays for anti-IgG and/or anti-C3d were reactive, whilst the tube assays were nonreactive. In conclusion, flow cytometric analysis is more sensitive than the tube assay for the detection of RBC-associated C3d. Further studies are needed to determine the correlation of C3d levels with clinical sequelae. |
| Databáze: | OpenAIRE |
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