Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy
| Autor: | Yves Emery, Christian Depeursinge, Benjamin Rappaz, Etienne Cuche, Pierre Marquet, Pierre J. Magistretti |
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| Rok vydání: | 2005 |
| Předmět: |
[MVD]
Wavefront Microscope Materials science business.industry Medical optics and biotechnology : Cell analysis Holography Phase (waves) Signal Instrumentation measurement and metrology : Phase measurement Atomic and Molecular Physics and Optics Instrumentation measurement and metrology : Metrology law.invention Optics Transmission (telecommunications) law Holography : Computer holography Digital holographic microscopy business Refractive index |
| Zdroj: | Optics Express. 13:9361 |
| ISSN: | 1094-4087 |
| DOI: | 10.1364/opex.13.009361 |
| Popis: | We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to lambda/1800, and the low acquisition time (~20micros) enable to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the integral refractive index and the cellular thickness (morphometry) from the measured phase shift is presented. Specifically, the method has been applied to study the dynamics of neurons in culture during a hypotonic stress. Such stress produces a paradoxical decrease of the phase which can be entirely resolved by applying the methodological approach described in this article; indeed the method allows to determine independently the thickness and the integral refractive index of cells. |
| Databáze: | OpenAIRE |
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