Production, purification and characterization of a proteolytic enzyme from Streptomyces sp. 2M21
| Autor: | Figen Zihnioglu, Irem Deniz, Fazilet Vardar-Sukan, E. Esin Hames, Suphi S. Oncel |
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| Přispěvatelé: | Bioengineering Department, Engineering Faculty, Manisa Celal Bayar UniversityMuradiye-Manisa, Turkey, Biochemistry Department, Faculty of Science, Ege UniversityBornova-Izmir, Turkey, Bioengineering Department, Engineering Faculty, Ege UniversityBornova-Izmir, Turkey, Ege Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
biology Keratinase purification Chemistry Proteolytic enzymes biology.organism_classification Biochemistry Streptomyces Catalysis Biodegradable composites Streptomyces sp bioreactor Enzyme Application areas parasitic diseases biology.protein Bioreactor characterization chicken feather Biotechnology |
| Popis: | EgeUn### The recent application studies on keratinase lead to new application areas for the enzyme to be used in novel industries such as pharmaceuticals and biodegradable composites. Thus, designing an economical and environmentally friendly keratinase production and purification with new features turned into an intriguing objective for its commercialization. In this study, keratinase enzyme was produced using a bioreactor of 5 L by a native Streptomyces sp. 2M21 and purified by ion exchange chromatography using step-wise gradient with 24.1-fold purification. Purified keratinase showed optimum activity at pH 9 and 30 degrees C. The enzyme was shown to be able to decompose different complex substrates such as chicken feather, wool and nail. Moreover, the addition of DTT enhanced the hydrolysis of wool and chicken feather to 3.4- and 3.2-fold, respectively. The potential of Streptomyces sp. to produce significant levels of keratinase using feathers as a substrate might establish cleaner applications for the production of high value-added products and degrading of keratin-containing wastes. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112M250]; Ege University Science-Technology and Research Center [EBILTEM-2013/BIL/020] This work was supported through research grants from The Scientific and Technological Research Council of Turkey (TUBITAK) with the project number of 112M250 and also Ege University Science-Technology and Research Center (EBILTEM-2013/BIL/020). |
| Databáze: | OpenAIRE |
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