Effects of Proanthocyanidins on Intestinal Motility Disturbance Following Intestinal Ischemia/Reperfusion
| Autor: | Xiao Zhang, Yu-Cheng Li, Qiang Song, De-Qi Cao, Lan Luo, Xiao-Zhen Dai, Zheng Yang, Wen-Si Hu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Pathology Antioxidant medicine.medical_treatment Drug Evaluation Preclinical Gastroenterology Superoxide dismutase Contractility Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Random Allocation 0302 clinical medicine Intestinal mucosa Ileum Laparotomy Internal medicine Malondialdehyde medicine Animals Proanthocyanidins Splanchnic Circulation bcl-2-Associated X Protein biology business.industry Caspase 3 Superoxide Dismutase Proanthocyanidin chemistry 030220 oncology & carcinogenesis Reperfusion Injury biology.protein 030211 gastroenterology & hepatology Surgery Ligation business Gastrointestinal Motility |
| Zdroj: | Journal of investigative surgery : the official journal of the Academy of Surgical Research. 29(6) |
| ISSN: | 1521-0553 |
| Popis: | To investigate the potential protective effects of Proanthocyanidins(PAs) on intestinal motility disturbance following intestinal ischemia/reperfusion (I/R).Male rats were divided into four groups: Sham, I/R, I/R+PA100 and I/R+PA200. Sham group underwent laparotomy without ligation, the others were subjected to intestinal ischemia for 1 h and reperfusion 4 h. Rats in the I/R+PA100 group received PAs (100 mg/kg/d) for 5 days prior to I/R, while rats in the I/R+PA200 group received PAs (200 mg/kg/d). After reperfusion, using an electrophysiology instrument measured ileal slow wave. Ileal specimens were obtained to determine contractility, tissue levels of Bax, Bcl-2, and Caspase-3 and evaluate histopathological changes. In addition, blood sample was obtained to determine serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels.Intestinal I/R caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, and hemorrhage. Both PAs treatment decreased mucosal pathological impairment in comparison with the I/R group (p.05) in light microscopic evaluations. In both PAs-treated groups, Bax and Caspase-3 expression were decreased compared to I/R group, while the Bcl-2 expression increased (p.05), which was similarly the case for serum SOD activity demonstrated significant enhance (p.05) and decline in MDA levels in comparison with I/R group (both p.05). Moreover, PAs treatment was more efficient in attenuating serum MDA levels of intestinal I/R (both p.05). And the contractile amplitude and frequency of slow wave in I/R+PA100 and I/R+PA200 groups were higher than I/R group (both p.05).PAs improve intestinal motility disturbance following intestinal I/R by alleviating oxidative stress and apoptosis. |
| Databáze: | OpenAIRE |
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