Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR–eIF4F pathway
| Autor: | Renata Cerna, Matthew S. Cook, Tereza Toralova, Denisa Jansova, Michal Kubelka, Jaroslava Supolikova, Jeong Su Oh, Martin Anger, Anna Danylevska, Andrej Susor, Radek Malik |
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| Rok vydání: | 2015 |
| Předmět: |
RNA Caps
Time Factors Nuclear Envelope Population Down-Regulation General Physics and Astronomy Biology Article Genomic Instability General Biochemistry Genetics and Molecular Biology Mice Eukaryotic initiation factor 4F Polar body medicine Protein biosynthesis Animals Humans RNA Messenger education Mammals education.field_of_study Multidisciplinary TOR Serine-Threonine Kinases Endoplasmic reticulum Translation (biology) General Chemistry Oocyte Chromosomes Mammalian Molecular biology Cell biology Meiosis medicine.anatomical_structure Eukaryotic Initiation Factor-4F Fertilization Protein Biosynthesis Oocytes Lamin Signal Transduction |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms7078 |
| Popis: | The fully grown mammalian oocyte is transcriptionally quiescent and utilizes only transcripts synthesized and stored during early development. However, we find that an abundant RNA population is retained in the oocyte nucleus and contains specific mRNAs important for meiotic progression. Here we show that during the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region that was previously surrounded the nucleus. These distinct translational hotspots are separated by endoplasmic reticulum and Lamin, and disappear following polar body extrusion. Chromosomal translational hotspots are controlled by the activity of the mTOR–eIF4F pathway. Here we reveal a mechanism that—following the resumption of meiosis—controls the temporal and spatial translation of a specific set of transcripts required for normal spindle assembly, chromosome alignment and segregation. Meiotic maturation of oocytes and early development of mammalian embryos is largely dependent on the translation of mRNAs stored in the oocyte. Here the authors uncover a population of mRNA retained in the oocyte nucleus whose translation is spatially and temporally regulated by the mTOR–eIF4F pathway during meiosis. |
| Databáze: | OpenAIRE |
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