Ex vivo studies of polymorphonuclear neutrophils from patients with early-onset-periodontitis (II). Chemiluminescence response analysis

Autor: P. Mouynet, B. Genetet, J. F. Michel, M. Delamaire, M. Yardin, M. C. Legoff
Rok vydání: 1994
Předmět:
Zdroj: Journal of Clinical Periodontology. 21:533-539
ISSN: 1600-051X
0303-6979
DOI: 10.1111/j.1600-051x.1994.tb01169.x
Popis: Previous studies have indicated that oxygen-free radicals may cause damage to the periodontal tissues. This study compared the luminol-dependant chemiluminescence response (after stimulation with either opsonized zymosan or Phorbol myristate acetate (PMA)) of peripheral blood polymorphonuclear leukocytes (PMN) isolated from human subjects with a healthy periodontium (n=7), gingivitis (n=8), adult periodontitis (n=8), or early-onset periodontitis (n=17). These results were also compared with those obtained in a larger reference group which consists of 50 subjects without infection or inflammation, selected on the basis of laboratory investigations. An enhanced response was defined as being 2 standard deviations above the reference group mean; a reduced response was. defined as being 2 standard deviations below this mean. Although PMN from patients with either gingivitis or periodontitis were often functionally activated (when compared to the PMN from the reference group), no significant differences could be found between the 4 groups, with regard to the chemiluminescence response means obtained in a basal state or after stimulation.
Databáze: OpenAIRE
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