Investigation of phenolic compounds at the leaves and shoots Arctostaphylos spp. and their antioxidant and antityrosinase activities
| Autor: | Arnold Alexeevich Shamilov, Daniil Nikolaevich Olennikov, Dmitryi Igorevich Pozdnyakov, Valentina Nikolaevna Bubenchikova, Ekaterina Robertovna Garsiya |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Natural Product Research. 36:6312-6317 |
| ISSN: | 1478-6427 1478-6419 |
| DOI: | 10.1080/14786419.2021.2025370 |
| Popis: | Comparative phytochemical and pharmacological analysis of leaves and shoots of Arctostaphylos uva-ursi (L.) Spreng. and endemic of Transcaucasia and North Caucasia Arctostaphylos caucasica Lipsch. was carried out. Phenolic compounds in methanol extracts were investigated by HPLC-PDA-ESI-MS. Pharmacological investigation was carried out at the models of generation of superoxide, DPPH, hydroxyl and nitrosyl radicals. The antityrosinase properties were evaluated in the reaction of tyrosinase inhibition using L -tyrosine as a substrate. Five new compounds were identified in the shoots and leaves of A. uva-ursi: 4,6-di-О-galloylglucose; 2,3-di-О-galloylglucose; 1,4,6-tri-О-galloylglucose; 1,2,4,6-tetra-О-galloylglucose; tetra-О-galloylhexose. Thirty-five phenolic compounds and two saponins were identified for the first time in A. caucasica. Water-alcohol extract (70% ethanol) from A. caucasica had the highest antiradical activity. Water extracts from both species had antityrosinase activity and possessed comparable efficiency (IC50 = 36.4 ± 1.2 µg/ml and IC50 = 35,5 ± 2 µg/ml, respectively). |
| Databáze: | OpenAIRE |
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