Evaluation of a standardized direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis in Kenya
| Autor: | F. Kibati, J.I. Githure, J. M. Kagai, D.K. Koech, Mbati Pa, G. Kirigi, Wasunna Km |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
business.industry 030231 tropical medicine Leishmaniasis medicine.disease World health 03 medical and health sciences Titer 0302 clinical medicine Infectious Diseases Visceral leishmaniasis Field screening 030225 pediatrics Direct agglutination test Internal medicine Immunology medicine Parasitology business Dried blood Third stage |
| Zdroj: | Annals of Tropical Medicine And Parasitology. 93:703-710 |
| ISSN: | 1364-8594 0003-4983 |
| DOI: | 10.1080/00034989957952 |
| Popis: | A prototype test kit being developed, by the World Health Organization (WHO), for the diagnosis of visceral leishmaniasis (VL) was evaluated in the Baringo district of Rift Valley province in Kenya. The screening of approximately 10 000 individuals for the signs of VL produced 305 suspected cases. These cases and 304 controls matched for sex and age (± 2 years) were then tested with the kit, which is based on a direct agglutination test (DAT). The evaluation was a three-stage process. The first stage, the field screening, involved screening filter-paper samples of dried blood from the suspects and controls at a DAT titre of 1:500. The second stage, the laboratory titration, involved screening of the same individuals by testing freshly eluted filter-paper samples at 1:500 to 1:2000 dilution. In the third stage, the full-scale titration, all samples that had been positive at 1:2000 were titrated at 1:500–1:512 000. All the suspects giving DAT titres of 1:2000 or higher were considered positive for VL. This ... |
| Databáze: | OpenAIRE |
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