Analysis of oxidative DNA damage and HPRT mutant frequencies in cancer patients before and after radiotherapy
| Autor: | Jos C. S. Kleinjans, P.A.E.L. Schilderman, Jos M.A de Jong, Irene J Welle, John J.M. Engelen, Ivo H. Zwingmann |
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| Přispěvatelé: | Gezondheidsrisico Analyse en Toxicologie, Klinische Genetica, Radiotherapie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism |
| Rok vydání: | 1999 |
| Předmět: |
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Hypoxanthine Phosphoribosyltransferase DNA damage Health Toxicology and Mutagenesis Breast Neoplasms Biology medicine.disease_cause Antioxidants chemistry.chemical_compound Neoplasms Genetics medicine Humans Deoxyguanosine Molecular Biology Aged Aged 80 and over Micronucleus Tests Cumulative dose Smoking Prostatic Neoplasms 8-Hydroxy-2'-deoxyguanosine DNA oxidation Middle Aged Molecular biology Bromodeoxyuridine chemistry 8-Hydroxy-2'-Deoxyguanosine Head and Neck Neoplasms Micronucleus test Female Micronucleus Oxidation-Reduction Biomarkers Oxidative stress DNA Damage |
| Zdroj: | Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 431(2), 361-369. Elsevier |
| ISSN: | 0027-5107 |
| Popis: | Department of Health Risk Analysis and Toxicology, Maastricht University, Netherlands.Various markers of radiation-induced DNA damage including DNA oxidation were investigated in peripheral lymphocytes of 23 cancer patients prior to and one week after receiving radiotherapy with a cumulative dose of 54-70 Gy. Exposure to ionizing radiation nonsignificantly increased the ratio 2'deoxy-7-dihydro-8-oxoguanosine/2'deoxyguanosine (8-oxodG/dG) from 1.73 x 10(-5) to 3.33 x 10(-5). Frequencies of micronuclei significantly (p = 0.0003) increased from 6.4 to 38.9 per 1000 cells. The frequency of hypoxanthine-guanine-phosphoribosyltransferase (HPRT) mutant lymphocytes measured as 6-thioguanine resistant variant cells by 5-bromodeoxyuridine labeling, was elevated eight-fold, from 4.7 x 10(-6) to 36.2 x 10(-6) (p = 0.008) after termination of the radiotherapy, thus showing a clear response to the radiation treatment. No correlation between levels of oxidative DNA damage and frequencies of HPRT mutant lymphocytes or micronuclei could be established. |
| Databáze: | OpenAIRE |
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