Hypermethylation of Chfr and hMLH1 in gastric noninvasive and early invasive neoplasias
| Autor: | Sumio Kawata, Zhe Jin, Kiyonari Ohmura, Naoyuki Homma, Teiichi Motoyama, Teiichiro Honda, Gen Tamura |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pathology medicine.medical_specialty Aging Ubiquitin-Protein Ligases Cell Cycle Proteins Biology Polymerase Chain Reaction Pathology and Forensic Medicine Loss of heterozygosity Stomach Neoplasms Chromosome instability Chromosomal Instability Neoplasms CHFR medicine Carcinoma Humans Neoplasm Invasiveness Poly-ADP-Ribose Binding Proteins Molecular Biology Adaptor Proteins Signal Transducing Aged Stomach Microsatellite instability Nuclear Proteins Cell Biology General Medicine DNA Methylation medicine.disease Genes p53 digestive system diseases Neoplasm Proteins Foveolar cell medicine.anatomical_structure Phenotype DNA methylation Cancer research Female Carrier Proteins MutL Protein Homolog 1 Microsatellite Repeats |
| Zdroj: | Virchows Archiv : an international journal of pathology. 446(2) |
| ISSN: | 0945-6317 |
| Popis: | Human tumors are genetically unstable, and the instability exists at two distinct levels—the chromosomal level and the nucleotide level. Chfr and hMLH1 hypermethylation, which may lead to chromosomal instability (CIN) and microsatellite instability (MSI), respectively, was analyzed in gastric noninvasive neoplasias (NIN, Padova international classification) and submucosal invasive adenocarcinomas and in their corresponding non-neoplastic gastric epithelia. Results were compared with microsatellite status, p53 immunoreactivity, and cellular phenotype. Hypermethylation of Chfr and hMLH1 was observed in: 10% (1/10) and 0% (0/10) of low-grade NIN (L-NIN); 63% (5/8) and 63% (5/8) of high-grade NIN, including suspicion for carcinoma without invasion (H-NIN); 36% (5/14) and 57% (8/14) of high-grade NIN, including carcinoma without invasion; and 35% (7/20) and 25% (5/20) of submucosal invasive adenocarcinomas, respectively. Hypermethylation was less frequent in L-NIN than H-NIN (P |
| Databáze: | OpenAIRE |
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