Construction andin VivoEfficacy of a Replication-Deficient Recombinant Adenovirus Encoding Murine Growth Hormone
| Autor: | Albert F. Parlow, Corinne M. Goldsmith, Yitzhak Marmary, Xinjun He, Lynnette K. Nieman, Martin Kriete, Kazuhito Satomura, Pamela Gehron Robey, Bruce J. Baum, Robert B. Wellner |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Submandibular Gland Radioimmunoassay Biology Virus Replication Injections Intramuscular Chromosomes Adenoviridae Cell Line law.invention Mice Endocrinology Pituitary Hormones Anterior In vivo law Internal medicine medicine Animals Rats Wistar Muscle Skeletal Growth factor Body Weight Gene Transfer Techniques Recombinant Proteins In vitro Rats DNA-Binding Proteins Somatropin Viral replication Cell culture Growth Hormone Recombinant DNA Transcription Factor Pit-1 Transcription Factors |
| Zdroj: | Endocrinology. 140:260-265 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | We have constructed a recombinant, replication-deficient, first-generation adenovirus-encoding mouse GH (mGH), AdCMVmGH. This virus directed mGH production from an epithelial cell line in vitro in a dose-dependent manner. When injected into the quadriceps muscle or submandibular ducts of mGH-deficient Snell dwarf mice, AdCMVmGH resulted in the production of significantly elevated serum mGH levels. Furthermore, after i.m. injection, dwarf mice increased in weight by 8% over 4 days and close to 100% by 30 days. When AdCMVmGH was administered to 3- to 4-week-old rats by i.v. injection to assess general metabolic responses, serum mGH, insulin-like growth factor 1, triglycerides and cholesterol levels were significantly elevated. AdCMVmGH should be a valuable experimental tool for the controlled, directed expression of mGH in preclinical mouse model studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|