Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills
| Autor: | Fabio Fava, Grazia Totaro, Noura Raddadi, Lucia Giacomucci |
|---|---|
| Přispěvatelé: | Raddadi, Noura, Giacomucci, Lucia, Totaro, Grazia, Fava, Fabio |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Geologic Sediments food.ingredient lcsh:QR1-502 Bioengineering Context (language use) Biodispersants Applied Microbiology and Biotechnology Petroleum Pollution lcsh:Microbiology Soybean oil 03 medical and health sciences chemistry.chemical_compound Surface-Active Agents food Marine bacteriophage RNA Ribosomal 16S Marinobacter Spectroscopy Fourier Transform Infrared Marinobacter sp 14. Life underwater Surface-active compounds biology Ecology Research Biodispersant Marine oil spills bioremediation Sequence Analysis DNA Biodegradation biology.organism_classification 6. Clean water Soybean Oil 030104 developmental biology Biodegradation Environmental Petroleum Surface-active compound chemistry 13. Climate action Environmental chemistry Ecotoxicity Stability Biotechnology |
| Zdroj: | Microbial Cell Factories Microbial Cell Factories, Vol 16, Iss 1, Pp 1-13 (2017) |
| ISSN: | 1475-2859 |
| Popis: | Background The application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, stable and effective under marine environment context. Biosurfactants from marine bacteria could be good candidates for the development of biodispersant formulations effective in marine environment. This study aimed at establishing a collection of marine bacteria able to produce surface-active compounds and evaluating the activity and stability of the produced compounds under conditions mimicking those found under marine environment context. Results A total of 43 different isolates were obtained from harbor sediments. Twenty-six of them produced mainly bioemulsifiers when glucose was used as carbon source and 16 were biosurfactant/bioemulsifiers producers after growth in the presence of soybean oil. Sequencing of 16S rRNA gene classified most isolates into the genus Marinobacter. The produced emulsions were shown to be stable up to 30 months monitoring period, in the presence of 300 g/l NaCl, at 4 °C and after high temperature treatment (120 °C for 20 min). The partially purified compounds obtained after growth on soybean oil-based media exhibited low toxicity towards V. fischeri and high capability to disperse crude oil on synthetic marine water. Conclusions To the best of our knowledge, stability characterization of bioemulsifiers/biosurfactants from the non-pathogenic marine bacterium Marinobacter has not been previously reported. The produced compounds were shown to have potential for different applications including the environmental sector. Indeed, their high stability in the presence of high salt concentration and low temperature, conditions characterizing the marine environment, the capability to disperse crude oil and the low ecotoxicity makes them interesting for the development of biodispersants to be used in combatting marine oil spills. Electronic supplementary material The online version of this article (10.1186/s12934-017-0797-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink