Detection of Antibodies Specific to an Antigenic Cell Wall Galactomannoprotein for Serodiagnosis of Aspergillus fumigatus Aspergillosis
| Autor: | Susanna K. P. Lau, Xiao-Yan Che, Che-Man Chan, Patrick C. Y. Woo, Andy S. P. Leung, Liang Cao, Kwok-Yung Yuen |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Antigens Fungal Melioidosis Guinea Pigs Enzyme-Linked Immunosorbent Assay Mycology Cross Reactions Biology Aspergillosis Sensitivity and Specificity Aspergillus fumigatus Penicilliosis Antibody Specificity medicine Animals Humans Aspergillus fumigatus - immunology skin and connective tissue diseases Antibodies Fungal Mycosis Aspergillosis - diagnosis Membrane Glycoproteins Antibodies Fungal - blood Fungal genetics bacterial infections and mycoses medicine.disease biology.organism_classification Antigens Fungal - genetics - immunology Recombinant Proteins Disease Models Animal Immunology Cryptococcosis Aspergilloma |
| Zdroj: | Journal of Clinical Microbiology. 40:2041-2045 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.40.6.2041-2045.2002 |
| Popis: | Aspergilloma and invasive aspergillosis are important opportunistic infections caused by Aspergillus species, among which Aspergillus fumigatus is the most common species associated with human disease. We developed an enzyme-linked immunosorbent assay (ELISA)-based antibody assay with Afmp1p, a purified recombinant antigenic cell wall galactomannoprotein of A. fumigatus. Evaluation of the test with guinea pig sera against A. fumigatus and other pathogenic fungi indicated that this assay was specific for A. fumigatus. Clinical evaluation revealed that the assay was 100% sensitive for patients with aspergilloma and 33.3% sensitive for patients with invasive aspergillosis. No false-positive results were found for serum samples from 80 healthy blood donors, 6 patients with typhoid fever, 4 patients with melioidosis, 20 patients with penicilliosis marneffei, 5 patients with candidiasis, and 4 patients with cryptococcosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Afmp1p antibody can be of significant value as a diagnostic for aspergillosis. published_or_final_version |
| Databáze: | OpenAIRE |
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