Surface PEGylation of Mesoporous Silica Nanorods (MSNR): Effect on loading, release, and delivery of mitoxantrone in hypoxic cancer cells
| Autor: | Stephanie L. Brock, David Oupický, Amit S. Wani, Ayat Abyad, Galbokka H. Layan Savithra, Shrey Kanvinde, Jing Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
musculoskeletal diseases
Cell Survival Surface Properties Science Antineoplastic Agents 02 engineering and technology Pharmacology 010402 general chemistry Hemolysis 01 natural sciences Article Polyethylene Glycols Drug Delivery Systems Cell Line Tumor Neoplasms medicine Animals Humans skin and connective tissue diseases IC50 Mitoxantrone Nanotubes Sheep Multidisciplinary Chemistry Mesoporous silica Silicon Dioxide 021001 nanoscience & nanotechnology medicine.disease Cell Hypoxia In vitro 0104 chemical sciences Drug Liberation Cell killing Cancer cell PEGylation Medicine 0210 nano-technology Porosity medicine.drug |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-02531-4 |
| Popis: | Mesoporous silica nanomaterials show great potential to deliver chemotherapeutics for cancer treatment. The key challenges in the development of injectable mesoporous silica formulations are colloidal instability, hemolysis and inefficient drug loading and release. In this study, we evaluated the effect of PEGylation of mesoporous silica nanorods (MSNR) on hemolysis, colloidal stability, mitoxantrone (MTX) loading, in vitro MTX release, and cellular MTX delivery under hypoxic conditions. We found that PEGylation prevented dose-dependent hemolysis in the concentrations studied (0–10 mg/ml) and improved colloidal stability of MSNR. A negative effect of PEGylation on MTX loading was observed but PEGylated MSNR (PMSNR) demonstrated increased MTX release compared to non-PEGylated particles. Under hypoxic conditions, a decrease in the IC50 of MTX and MTX-loaded MSNR was observed when compared to normoxic conditions. These results showed that MSNR could deliver the chemotherapeutic agent, MTX to tumor cells and induce effective cell killing. However, the effect of PEGylation needs to be carefully studied due to the observed adverse effect on drug loading. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|