Direct and Indirect Visualization of Bacterial Effector Delivery into Diverse Plant Cell Types during Infection
| Autor: | Tania Y. Toruño, Elizabeth Henry, Alain Jauneau, Laurent Deslandes, Gitta Coaker |
|---|---|
| Přispěvatelé: | Department of Plant Pathology, Cornell University, Institut Fédératif de Recherche 3450, Plateforme Imagerie TRI-Genotoul, Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique (CNRS), UPS, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), USDA National Institute of Food and Agriculture predoctoral fellowship : 2014-67011-21563, National Institutes of Health : RO1GM092772, ANR : ANR-15-CE20-0016-01, Laboratoire d'Excellence (LABEX) TULIP : ANR-10-LABX-41, région Occitanie/Pyrenees-Mediterranee (PRISM-Project), Cornell University [New York], Plateforme Imagerie, Université Toulouse III - Paul Sabatier (UT3), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
genetic structures Virulence Factors Green Fluorescent Proteins Arabidopsis Pseudomonas syringae Ralstonia Plant Science Biology In Brief Microbiology Type three secretion system Green fluorescent protein Epitopes 03 medical and health sciences Bacterial Proteins Solanum lycopersicum infection de la plante Plant Cells Tobacco plant breeding [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Arabidopsis thaliana pathologie végétale Pathogen Plant Diseases Ralstonia solanacearum Vegetal Biology défense cellulaire Effector Microbiology and Parasitology fungi food and beverages Cell Biology Plants Genetically Modified biology.organism_classification Microbiologie et Parasitologie eye diseases Molecular Imaging Plant Leaves [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology 030104 developmental biology Host-Pathogen Interactions Breakthrough Reports protection des cultures Biologie végétale amélioration des plantes |
| Zdroj: | Plant Cell Plant Cell, American Society of Plant Biologists, 2017, 29 (7), pp.1555-1570. ⟨10.1105/tpc.17.00027⟩ The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2017, 29 (7), pp.1555-1570. ⟨10.1105/tpc.17.00027⟩ Plant Cell 7 (29), 1555-1570. (2017) |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.17.00027 |
| Popis: | To cause disease, diverse pathogens deliver effector proteins into host cells. Pathogen effectors can inhibit defense responses, alter host physiology, and represent important cellular probes to investigate plant biology. However, effector function and localization have primarily been investigated after overexpression in planta. Visualizing effector delivery during infection is challenging due to the plant cell wall, autofluorescence, and low effector abundance. Here, we used a GFP strand system to directly visualize bacterial effectors delivered into plant cells through the type III secretion system. GFP is a beta barrel that can be divided into 11 strands. We generated transgenic Arabidopsis thaliana plants expressing GFP1-10 (strands 1 to 10). Multiple bacterial effectors tagged with the complementary strand 11 epitope retained their biological function in Arabidopsis and tomato (Solanum lycopersicum). Infection of plants expressing GFP1-10 with bacteria delivering GFP11-tagged effectors enabled direct effector detection in planta. We investigated the temporal and spatial delivery of GFP11-tagged effectors during infection with the foliar pathogen Pseudomonas syringae and the vascular pathogen Ralstonia solanacearum. Thus, the GFP strand system can be broadly used to investigate effector biology in planta. |
| Databáze: | OpenAIRE |
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