Salivary Microbiological and Gingival Health Status Evaluation of Adolescents With Overweight and Obesity: A Cluster Analysis

Autor: Kelly Guedes de Oliveira Scudine, Luana de Sales Leite, Paula Midori Castelo, Darlle Santos Araujo, Thaís Manzano Parisotto, Marlise I. Klein, Caroline M. Ferreira, Matheus Moreira Perez, Fernando Luiz Affonso Fonseca
Přispěvatelé: Universidade Estadual de Campinas (UNICAMP), Universidade Estadual Paulista (Unesp), São Francisco University, Universidade Federal de São Paulo (UNIFESP), ABC Medical School
Rok vydání: 2020
Předmět:
Zdroj: Frontiers in Pediatrics
Frontiers in Pediatrics, Vol 8 (2020)
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 2296-2360
Popis: Made available in DSpace on 2020-12-12T01:35:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-31 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Given the high prevalence of obesity in children and adolescents, the investigation of early markers is of clinical importance to better manage this condition. Thus, the aim was to evaluate the cross-sectional relationship between salivary microbiota, gingival health status, and excess weight in adolescents. A total of 248 students (14–17 y; 119 girls) were included, free of caries lesions and periodontal pockets. Physical examination included measures of height, weight, and body fat percentage (%BF). Oral examination was performed to gather information on dental (DMFT index) and gingival health status. Unstimulated saliva was submitted to qPCR reactions to quantify Streptococcus mutans, Porphyromonas gingivalis, Bifidobacteria, and Streptococcus pneumoniae percentages and the NFKappaB expression. Two-way ANOVA was applied considering group (normal-weight/overweight/obesity) and sex factors, in addition to cluster analysis. Group effect was significant for %S. mutans (partial eta2 = 0.20; p < 0.001) and %Bifidobacteria (partial eta2 = 0.19; p < 0.001), with overweight and obesity groups showing the highest levels compared to normal-weight ones, with no significant sex effect. There was no difference in the frequency of gingivitis, P. gingivalis, and S. pneumoniae percentages or NFKappaB expression between groups. Cluster analysis generated three clusters according to body fat accumulation: “Higher %BF,” “Moderate %BF,” and “Lower %BF.” “Higher %BF” cluster was characterized by higher body fat percentage and higher salivary %Bifidobacteria, while cluster “Lower %BF” was characterized by lower body fat percentage and lower frequency of gingivitis (“Moderate %BF” cluster was the contrast). According to nutritional status, a difference in salivary S. mutans and Bifidobacteria percentages was found, with overweight or obesity adolescents showing the highest percentages than normal-weight ones. Besides, a positive relationship between body fat accumulation and Bifidobacteria count was observed, indicating a possible interaction between oral bacteria communities and weight gain. Department of Pediatric Dentistry Universidade Estadual de Campinas (UNICAMP Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry Laboratory of Molecular Biology of Microorganisms São Francisco University Department of Pharmaceutical Sciences Universidade Federal de São Paulo (UNIFESP Department of Pathology ABC Medical School Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry CAPES: 001 FAPESP: 2014/24804-4 FAPESP: 2017/26400-6
Databáze: OpenAIRE
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