Gene organization and transcription of a late-expressed region of a Lactococcus lactis phage
| Autor: | A. L. S. Lerayer, R. Valyasevi, Marie-Christine Chopin, Stanislav Dusko Ehrlich, Ricardo Parreira |
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| Přispěvatelé: | Unité de recherche Génétique Microbienne (UGM), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Rok vydání: | 1996 |
| Předmět: |
Phage display
Transcription Genetic Sequence analysis viruses Phagemid Molecular Sequence Data Gene Expression Biology Microbiology Genome 03 medical and health sciences Transcription (biology) Consensus sequence LACTOCOCCUS LACTIS Bacteriophages ORFS [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences Base Sequence 030306 microbiology Sequence Analysis DNA Molecular biology Open reading frame [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology DNA Viral Research Article |
| Zdroj: | Scopus-Elsevier Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 1996, 178 (21), pp.6158-6165 Europe PubMed Central |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.178.21.6158-6165.1996 |
| Popis: | The lactococcal phage bIL41 belongs to the small isometric-headed phages of the 936 quasi-species and is resistant to the abortive infection determined by abiB. A 10.2-kb segment from this phage, in which late transcription is initiated, has been sequenced. Thirteen open reading frames (ORFs) organized in one transcriptional unit have been identified. The location of two of them and the structural features of the proteins they code for are evocative of terminase subunits. Five other ORFs specify proteins which are highly homologous to structural proteins from the closely related phage F4-1. By comparing the phage bIL41 sequence with partial sequences available for four related phages, we were able to deduce a chimerical phage map covering the middle- and a large part of the late-expressed regions. Phages from this quasi-species differ by the insertion or deletion of either 1 to about 400 bp in noncoding regions or an entire ORF. Transcription was initiated 9 min after infection at a promoter with a -10 but no -35 consensus sequence. Synthesis of a phage activator protein was needed for initiation of transcription. A large 16-kb transcript covering all of the late-expressed region of the genome was synthesized. This transcript gave rise to smaller units. One of these units most probably resulted from a RNase E processing. |
| Databáze: | OpenAIRE |
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