ADP-glucose pyrophosphorylase gene plays a key role in the quality of corm and yield of cormels in gladiolus
| Autor: | Juanjuan Sui, Fengqin Zhang, Xionghui Zhong, Shanshan Seng, Chao Liu, Mingfang Yi, Junna He, Chenyu Wu, Jian Wu, Chen Liu, Benhe Gong |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Starch Mutant Biophysics Glucose-1-Phosphate Adenylyltransferase Corm 01 natural sciences Biochemistry Iridaceae 03 medical and health sciences chemistry.chemical_compound Arabidopsis Botany Gene silencing Tissue Distribution Gladiolus Molecular Biology biology food and beverages Cell Biology biology.organism_classification Plant Leaves Plant Tubers Horticulture 030104 developmental biology chemistry Organ Specificity 010606 plant biology & botany |
| Zdroj: | Biochemical and Biophysical Research Communications. 474:206-212 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2016.04.103 |
| Popis: | Starch is the main storage compound in underground organs like corms. ADP-glucose pyrophosphorylase (AGPase) plays a key role in regulating starch biosynthesis in storage organs and is likely one of the most important determinant of sink strength. Here, we identify an AGPase gene (GhAGPS1) from gladiolus. The highest transcriptional levels of GhAGPS1 were observed in cormels and corms. Transformation of GhAGPS1 into Arabidopsis rescued the phenotype of aps1 mutant. Silencing GhAGPS1 in gladiolus corms by virus-induced gene silencing (VIGS) decreased the transcriptional levels of two genes and starch content. Transmission electron microscopy analyses of leaf and corm sections confirmed that starch biosynthesis was inhibited. Corm weight and cormel number reduced significantly in the silenced plants. Taken together, these results indicate that inhibiting the expression of AGPase gene could impair starch synthesis, which results in the lowered corm quality and cormel yield in gladiolus. |
| Databáze: | OpenAIRE |
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