Regulation of prostate cell collagen receptors by malignant transformation
| Autor: | Heikki Hiekkanen, Jyrki Heino, Markku Kallajoki, Kalle Alanen, Camilla Nylund, Donald Gullberg, Janika Lehtonen, Tuomas Mirtti, Liisa Nissinen |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Integrins Receptors Collagen Integrin Biology medicine.disease_cause Collagen receptor Prostate cancer Internal medicine medicine Humans Receptor Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Carcinoma Prostate Prostatic Neoplasms Sarcoma medicine.disease Immunohistochemistry Androgen receptor Endocrinology Cell Transformation Neoplastic Oncology Tumor progression Receptors Androgen Cancer cell Cancer research biology.protein Carcinogenesis |
| Zdroj: | International journal of cancer. 118(4) |
| ISSN: | 0020-7136 |
| Popis: | Cell adhesion receptors, including the integrin-type collagen receptors (alpha1beta1, alpha2beta1, alpha10beta1 and alpha11beta1) participate in cancer progression and invasion. Quantitative RT-PCR indicated that all 4 receptors are abundantly expressed in sarcoma-derived cell lines, whereas most carcinoma-derived cells express alpha1beta1 and alpha2beta1 only. This was surprising because alpha11beta1 has been connected previously to the progression of lung adenocarcinomas. To test the hypothesis that alpha11 expression may not persist in cultured cancer cells we analyzed fresh tissue samples of 104 total prostatectomies, keeping in mind that prostate cancer cell lines showed negligible alpha11 mRNA levels. In prostate alpha2 expression was significantly lower in poorly differentiated carcinomas when compared to benign lesions (p = 0.0331). In immunohistochemistry the protein levels of alpha2 integrin decreased significantly (p = 0.0001) and the protein levels of alpha11 subunit increased significantly (p = 0.029) with the increasing grade of carcinoma. Thus alpha11beta1 may replace alpha2beta1 during tumor progression. Our observations support the idea that alpha11beta1 may be expressed in tumors but the corresponding cell lines may lose the expression of this integrin. Previous studies have shown that in cell culture androgen receptor (AR) controls alpha2beta1 expression. We measured AR mRNA levels and the number of AR positive nuclei in the prostate samples and the results showed a significant correlation between alpha2beta1 and AR. Androgen receptors may control the mechanisms regulating integrin expression in prostate. |
| Databáze: | OpenAIRE |
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