Complete-genome sequencing elucidates outbreak dynamics of CA-MRSA USA300 (ST8-spa t008) in an academic hospital of Paramaribo, Republic of Suriname
| Autor: | Alexander W. Friedrich, Amadu Juliana, Sandra Hermelijn, John E. Degener, Viktoria Akkerboom, Artur J. Sabat, Hajo Grundmann |
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| Přispěvatelé: | Microbes in Health and Disease (MHD) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Methicillin-Resistant Staphylococcus aureus GENES TRANSMISSION 030106 microbiology Single-nucleotide polymorphism Biology Genome Polymorphism Single Nucleotide Article Disease Outbreaks 03 medical and health sciences Plasmid MOLECULAR EPIDEMIOLOGY INFECTION Pulsed-field gel electrophoresis FIELD GEL-ELECTROPHORESIS Humans Phylogeny Aged Genetics Whole genome sequencing Multidisciplinary Suriname Molecular epidemiology Whole Genome Sequencing RESISTANT STAPHYLOCOCCUS-AUREUS Outbreak Infant Middle Aged Staphylococcal Infections TANDEM DUPLICATION EVOLUTION BACTERIA Multilocus sequence typing SPREAD Genome Bacterial |
| Zdroj: | Scientific Reports Scientific Reports, 7:41050, 1-12. Nature Publishing Group |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep41050 |
| Popis: | We report the investigation of an outbreak situation of methicillin-resistant Staphylococcus aureus (MRSA) that occurred at the Academic Hospital Paramaribo (AZP) in the Republic of Suriname from April to May 2013. We performed whole genome sequencing with complete gap closure for chromosomes and plasmids on all isolates. The outbreak involved 12 patients and 1 healthcare worker/nurse at the AZP. In total 24 isolates were investigated. spa typing, genome-wide single nucleotide polymorphism (SNP) analysis, ad hoc whole genome multilocus sequence typing (wgMLST), stable core genome MLST (cgMLST) and in silico PFGE were used to determine phylogenetic relatedness and to identify transmission. Whole-genome sequencing (WGS) showed that all isolates were members of genomic variants of the North American USA300 clone. However, WGS revealed a heterogeneous population structure of USA300 circulating at the AZP. We observed up to 8 SNPs or up to 5 alleles of difference by wgMLST when the isolates were recovered from different body sites of the same patient or if direct transmission between patients was most likely. This work describes the usefulness of complete genome sequencing of bacterial chromosomes and plasmids providing an unprecedented level of detail during outbreak investigations not being visible by using conventional typing methods. |
| Databáze: | OpenAIRE |
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