Continuous Label-Free Electronic Discrimination of T Cells by Activation State
| Autor: | Tarek M. Fahmy, Shari Yosinski, Rabib Chaudhury, Mark A. Reed, Zachary Kobos, Jung Seok Lee, Patrick Han |
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| Rok vydání: | 2020 |
| Předmět: |
Electrophoresis
Cellular immunity T-Lymphocytes T cell Microfluidics General Physics and Astronomy Cell Separation 02 engineering and technology Lymphocyte Activation 010402 general chemistry 01 natural sciences Immune system Antigen Immunity Coulter counter medicine Cytotoxic T cell General Materials Science Chemistry General Engineering Dielectrophoresis 021001 nanoscience & nanotechnology 0104 chemical sciences Cell biology medicine.anatomical_structure Biological Assay Electronics 0210 nano-technology |
| Zdroj: | ACS Nano. 14:8646-8657 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.0c03018 |
| Popis: | The sensitivity and speed with which the immune system reacts to host disruption is unrivaled by any detection method for pathogenic biomarkers or infectious signatures. Engagement of cellular immunity in response to infections or cancer is contingent upon activation and subsequent cytotoxic activity by T cells. Thus, monitoring T cell activation can reliably serve as a metric for disease diagnosis as well as therapeutic prognosis. Rapid and direct quantification of T cell activation states, however, has been hindered by challenges associated with antigen target identification, labeling requirements, and assay duration. Here we present an electronic, label-free method for simultaneous separation and evaluation of T cell activation states. Our device utilizes a microfluidic design integrated with nanolayered electrode structures for dielectrophoresis (DEP)-driven discrimination of activated vs naive T cells at single-cell resolution and demonstrates rapid ( |
| Databáze: | OpenAIRE |
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