Ghrelin suppresses migration of macrophages via inhibition of ROCK2 under chronic intermittent hypoxia

Autor: Hao Tong, Hong Chen, Man Zhang, Jianfeng Du, Siying Zhang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
Medicine (General)
macrophage migration
Coronary Artery Disease
Biochemistry
Pre-Clinical Research Report
03 medical and health sciences
Mice
0302 clinical medicine
R5-920
ROCK2
Cell Movement
Internal medicine
chronic intermittent hypoxia
Chronic intermittent hypoxia
Medicine
Animals
Humans
Hypoxia
obstructive sleep apnea
Sleep Apnea
Obstructive
rho-Associated Kinases
business.industry
Macrophages
Biochemistry (medical)
Cell Biology
General Medicine
medicine.disease
Atherosclerosis
Coronary heart disease
Cell Hypoxia
Ghrelin
Rats
Obstructive sleep apnea
Disease Models
Animal
030104 developmental biology
Endocrinology
RAW 264.7 Cells
Chronic Disease
Endothelium
Vascular
business
030217 neurology & neurosurgery
Injections
Intraperitoneal
Signal Transduction
Zdroj: The Journal of International Medical Research
Journal of International Medical Research, Vol 48 (2020)
ISSN: 1473-2300
0300-0605
Popis: Objectives Migration of macrophages and atherosclerosis result in various diseases, including coronary heart disease. This study aimed to clarify the roles that ghrelin and Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) play in migration of macrophages under chronic intermittent hypoxia (CIH). Methods A rat model of CIH was constructed and changes in ghrelin and ROCK2 protein expression were measured by western blot assay. The migratory ability of macrophages was determined by the transwell assay. Hematoxylin and eosin staining was applied to detect the changes in intima-media thickness. Results We found that CIH enhanced migration of macrophages, and this effect was attenuated by exogenous ghrelin. Additionally, the facilitative effect of CIH on migration of macrophages was strengthened or decreased by upregulation or downregulation of ROCK2, respectively. This phenomenon indicated that ROCK2 was involved in CIH-induced migration in macrophages. Furthermore, western blot and transwell assays showed that ghrelin inhibited CIH-induced migration via ROCK2 suppression in macrophages. Conclusions In summary, the present study shows that ghrelin inhibits CIH-induced migration via ROCK2 suppression in macrophages. Our research may help lead to identifying a new molecular mechanism for targeted therapy of atherosclerosis and its associated coronary artery diseases under intermittent hypoxia.
Databáze: OpenAIRE
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