Ghrelin suppresses migration of macrophages via inhibition of ROCK2 under chronic intermittent hypoxia
Autor: | Hao Tong, Hong Chen, Man Zhang, Jianfeng Du, Siying Zhang |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty Medicine (General) macrophage migration Coronary Artery Disease Biochemistry Pre-Clinical Research Report 03 medical and health sciences Mice 0302 clinical medicine R5-920 ROCK2 Cell Movement Internal medicine chronic intermittent hypoxia Chronic intermittent hypoxia Medicine Animals Humans Hypoxia obstructive sleep apnea Sleep Apnea Obstructive rho-Associated Kinases business.industry Macrophages Biochemistry (medical) Cell Biology General Medicine medicine.disease Atherosclerosis Coronary heart disease Cell Hypoxia Ghrelin Rats Obstructive sleep apnea Disease Models Animal 030104 developmental biology Endocrinology RAW 264.7 Cells Chronic Disease Endothelium Vascular business 030217 neurology & neurosurgery Injections Intraperitoneal Signal Transduction |
Zdroj: | The Journal of International Medical Research Journal of International Medical Research, Vol 48 (2020) |
ISSN: | 1473-2300 0300-0605 |
Popis: | Objectives Migration of macrophages and atherosclerosis result in various diseases, including coronary heart disease. This study aimed to clarify the roles that ghrelin and Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) play in migration of macrophages under chronic intermittent hypoxia (CIH). Methods A rat model of CIH was constructed and changes in ghrelin and ROCK2 protein expression were measured by western blot assay. The migratory ability of macrophages was determined by the transwell assay. Hematoxylin and eosin staining was applied to detect the changes in intima-media thickness. Results We found that CIH enhanced migration of macrophages, and this effect was attenuated by exogenous ghrelin. Additionally, the facilitative effect of CIH on migration of macrophages was strengthened or decreased by upregulation or downregulation of ROCK2, respectively. This phenomenon indicated that ROCK2 was involved in CIH-induced migration in macrophages. Furthermore, western blot and transwell assays showed that ghrelin inhibited CIH-induced migration via ROCK2 suppression in macrophages. Conclusions In summary, the present study shows that ghrelin inhibits CIH-induced migration via ROCK2 suppression in macrophages. Our research may help lead to identifying a new molecular mechanism for targeted therapy of atherosclerosis and its associated coronary artery diseases under intermittent hypoxia. |
Databáze: | OpenAIRE |
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