The unique GGA clathrin adaptor of Drosophila melanogaster is not essential
| Autor: | Anne M. Ilvarsonn, Joel C. Eissenberg, Shan Luan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Genetic Screens Gene Expression lcsh:Medicine RNA interference Molecular Cell Biology Drosophila Proteins RNA Small Interfering lcsh:Science Genetics Gene knockdown Multidisciplinary biology Drosophila Melanogaster Homozygote Signal transducing adaptor protein Chloroquine Animal Models Cellular Structures Survival Rate Phenotype Gene Knockdown Techniques Clathrin adaptor proteins Epigenetics Membranes and Sorting Female Drosophila melanogaster Drosophila Protein Research Article trans-Golgi Network Endosome Endosomes Clathrin Molecular Genetics Model Organisms Autophagy Animals Biology Hemizygote fungi lcsh:R biology.organism_classification Adaptor Proteins Vesicular Transport Subcellular Organelles Starvation Mutation biology.protein lcsh:Q Gene Function Lysosomes Animal Genetics |
| Zdroj: | PLoS ONE, Vol 7, Iss 9, p e45163 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The Golgi-localized, γ-ear-containing, ARF binding proteins (GGAs) are a highly conserved family of monomeric clathrin adaptor proteins implicated in clathrin-mediated protein sorting between the trans-Golgi network and endosomes. GGA RNAi knockdowns in Drosophila have resulted in conflicting data concerning whether the Drosophila GGA (dGGA) is essential. The goal of this study was to define the null phenotype for the unique Drosophila GGA. We describe two independently derived dGGA mutations. Neither allele expresses detectable dGGA protein. Homozygous and hemizygous flies with each allele are viable and fertile. In contrast to a previous report using RNAi knockdown, GGA mutant flies show no evidence of age-dependent retinal degeneration or cathepsin missorting. Our results demonstrate that several of the previous RNAi knockdown phenotypes were the result of off-target effects. However, GGA null flies are hypersensitive to dietary chloroquine and to starvation, implicating GGA in lysosomal function and autophagy. |
| Databáze: | OpenAIRE |
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