Induction of Retinal Progenitors and Neurons from Mammalian Müller Glia under Defined Conditions
| Autor: | Meixia Zhang, Jack Jiagang Zhao, John Quach, Yuanchao Xue, Shaochen Chen, Sherrina Patel, Sheng Ding, Nicole Sfeir, Xiang-Dong Fu, Hong Ouyang, Jing Luo, Kang Zhang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Giant retinal ganglion cells
Biology Real-Time Polymerase Chain Reaction Biochemistry Retinal ganglion Retina Mice chemistry.chemical_compound medicine Animals Molecular Biology DNA Primers Neurons Base Sequence Reverse Transcriptase Polymerase Chain Reaction Stem Cells Intrinsically photosensitive retinal ganglion cells Cell Differentiation Retinal Cell Biology Anatomy Cell biology Transplantation medicine.anatomical_structure nervous system chemistry Neuroglia sense organs Muller glia Photoreceptor Cells Vertebrate |
| Zdroj: | Journal of Biological Chemistry. 289:11945-11951 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m113.532671 |
| Popis: | Vision impairment caused by loss of retinal neurons affects millions of people worldwide, and currently, there is no effective treatment. Müller glia of mammalian retina may represent an under-recognized and potential source for regeneration of a wide range of retinal cell types, including retinal ganglion cells and photoreceptors. Here, we demonstrated that mouse Müller glia cells have the capacity to be reprogrammed into the retinal neuronal cell fate and are competent to give rise to photoreceptors under a defined culture condition. Inactivation of p53 released proliferation restriction of Müller glia and significantly enhanced the induction of retinal progenitor from Müller glia in culture. Moreover, following the ocular transplantation, the Müller glia-derived progenitors were differentiated toward the fates of photoreceptors and retinal ganglion cells. Together, these results demonstrate the feasibility of using Müller glia as a potential source for retinal repair and regeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|