Effects of cocaine on c-fos and preprodynorphin mRNA levels in intact and ovariectomized Fischer rats
| Autor: | Linda I. Perrotti, Juliet Chin, Shirzad Jenab, Vanya Quinones-Jenab, Scott J. Russo, Tipyamol Niyomchai, Eugene D. Festa |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class Ovariectomy Estrous Cycle Dynorphin Biology Dynorphins c-Fos Drug Administration Schedule Cocaine-Related Disorders Cocaine Internal medicine medicine Animals RNA Messenger Protein Precursors Gonadal Steroid Hormones Progesterone Sex Characteristics Behavior Animal General Neuroscience Estrogens Rats Inbred F344 Rats Up-Regulation Neostriatum Endocrinology Gene Expression Regulation Opioid Estrogen biology.protein Ovariectomized rat Solution hybridization Female Proto-Oncogene Proteins c-fos Immediate early gene medicine.drug Hormone |
| Zdroj: | Brain Research Bulletin. 58:295-299 |
| ISSN: | 0361-9230 |
| DOI: | 10.1016/s0361-9230(02)00793-1 |
| Popis: | Psychostimulants such as cocaine have been shown to regulate c -fos and opioid gene expression in male rats. However, little information is available on cocaine effects in female rats or how the ovarian hormones, estrogen and progesterone, modulate these effects. In this study we used quantitative solution hybridization assays to measure c -fos and preprodynorphin (PDYN) mRNA levels after cocaine administration in the caudate/putamen of intact male and female rats or ovariectomized (OVX) female rats that were pretreated with vehicle, estrogen and/or progesterone. The c -fos mRNA levels were increased in intact male and female rats after 30 min or 3 h of one single cocaine injection and after 14 days of single daily cocaine injections. The c -fos mRNA levels were also increased after 30 min of a single cocaine injection in OVX female rats that were treated with vehicle, estrogen and/or progesterone. The PDYN mRNA levels did not change after 30 min, 3 h or 14 days in intact male or female rats. However, PDYN mRNA levels were increased in the caudate/putamen of OVX female rats pretreated with vehicle or a combination of estrogen and progesterone but not in OVX female rats that were pretreated with either estrogen or progesterone alone. Our data suggest hormonal regulation of cocaine effects on PDYN mRNA levels which may modulate cocaine-induced behaviors in female rats. |
| Databáze: | OpenAIRE |
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