Preparation of Curcumin-Containing α-, β-, and γ-Cyclodextrin/Polyethyleneglycol-Conjugated Gold Multifunctional Nanoparticles and Their in Vitro Cytotoxic Effects on A549 Cells
| Autor: | Akihiro Hoshikawa, Tetsuya Ozeki, Kaori Fukushige, Mizuki Nagira, Tatsuaki Tagami, Masafumi Tane |
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| Rok vydání: | 2018 |
| Předmět: |
Curcumin
Cell Survival Drug Compounding Metal Nanoparticles Pharmaceutical Science Antineoplastic Agents 02 engineering and technology Conjugated system Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Humans Pharmacology chemistry.chemical_classification Cyclodextrins Cyclodextrin Biological Transport General Medicine Photothermal therapy 021001 nanoscience & nanotechnology chemistry A549 Cells Colloidal gold 030220 oncology & carcinogenesis PEGylation Nanomedicine Gold Nanocarriers 0210 nano-technology Nuclear chemistry |
| Zdroj: | Biological and Pharmaceutical Bulletin. 41:908-914 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b18-00010 |
| Popis: | Gold nanoparticles (GNPs) have promising properties such as photothermal effects and could be useful for imaging and as multifunctional nanocarriers for various drugs. In this study, we synthesized polyethyleneglycol (PEG)-grafted GNPs and conjugated them with cyclodextrin (CD) to incorporate curcumin. Curcumin has anticancer effects but its therapeutic application is limited due to poor water solubility. Three types of CDs (α-, β-, and γ-CDs) were conjugated with PEGylated GNPs and the curcumin-containing CD/PEG-conjugated GNPs (cur-CD-GNPs) were characterized. Transmission electron microscopy and dynamic light scattering results showed that these cur-CD-GNPs have a small gold nanocore (approximately 5 nm) and the average size of the three cur-CD-GNPs was approximately 25-35 nm. Curcumin was efficiently incorporated into the β-CD solution and the loading efficiency of curcumin in β-CD-GNPs was the highest of the three types of CD-GNPs prepared. The cytotoxic effect of cur-CD-GNPs was investigated using a human lung cancer cell line. All cur-CD-GNPs exhibited cytotoxic effects comparable to that of curcumin solution and CD-GNPs without curcumin were not cytotoxic. These results suggest that cur-CD-GNPs may be a useful multifunctional nanomedicine, although in vivo investigations are required. |
| Databáze: | OpenAIRE |
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