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Purpose The antinuclear antibody (ANA) test, despite all the downsides, is still considered and used as the gold standard technique amongst all the other options to diagnose autoimmune diseases. Our aim was to investigate the positivity rate and pattern distribution of ANA test results as well as the relationship between test results and certain demographic characteristics of patients. Methods In this cross-sectional study, the ANA test results of 3469 adult patients who were diagnosed and classified by rheumatologists, other clinicians were evaluated between JanuaryMay 2022 in Dokuz Eylul University Hospital Immunology Laboratory. All samples were studied with indirect immunofluorescence antibody (IIFA) assay on HEp-2 cells (HEp-2-IIFA) and staining patterns were evaluated. In the analysis of the data, descriptive statistics [mean (sd), median (interquartile range (IQR)), percent (%)], chi-square test and Mann-Whitney u test were used according to their suitability. Statistical significance was quantified at the p Results The median age of the patients included in the study was 52.00 (39.00-65.00) years and 65.9% (n=2286) of the research group consisted of women. When the anti-cell (AC) autoantibodies were evaluated according to The Internatinal Consensus on ANA Patterns; ANA positivity was detected in 59% (n=2048) of the patients, while AC-0 (negative) was found in 41.0% (n=1421) of the patients. "AC-0","AC-4,5","AC-2","AC-8,9,10","AC-21","AC-19,20" patterns were the most frequent patterns observed. ANA positivity was significantly higher in females than males (p Conclusions Nearly three-fifths of the patients studied were HEp-2-IIFA-positive. Individuals aged 50 and over and female patients had more positive results. As a result, female patients and elderly patients may need to be evaluated more carefully in terms of ANA positivity. |
