Regulation of colon gene expression by vitamin B6 supplementation
| Autor: | Norihisa Kato, Atsuko Hirata, Tomomi Ohata, Keigo Toya, Yohei Sanada, Noriyuki Yanaka |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Colorectal cancer Colon CD8 Antigens Azoxymethane Down-Regulation Biology CCL8 chemistry.chemical_compound Mice Aberrant Crypt Foci Internal medicine Gene expression medicine Animals Chemokine CCL8 RNA Messenger Carcinogen Cell Proliferation Oligonucleotide Array Sequence Analysis Regulation of gene expression Mice Inbred ICR Cell growth medicine.disease digestive system diseases Vitamin B 6 Gene Expression Regulation Neoplastic Endocrinology chemistry Colonic Neoplasms Dietary Supplements Food Science Biotechnology Aberrant crypt foci |
| Zdroj: | Molecular nutritionfood research. 56(4) |
| ISSN: | 1613-4133 |
| Popis: | Scope Previous studies have shown that vitamin B6 supplementation suppresses the development of colonic aberrant crypt foci (ACF), precursor lesions of colon cancer, and cell proliferation in mice receiving the colonic carcinogen, azoxymethane (AOM). This study investigated the molecular mechanism of these effects of dietary vitamin B6. Methods and results To date, the mechanism by which ACFs develop is not yet fully understood. In a search for factors that play a critical role during ACF development, we examined colon gene expression during early stage of ACF development in AOM-treated mice using DNA microarray analysis. AOM treatment significantly upregulated mRNA closely related to mast cell and cytotoxic T-cell activity. This study also investigated the effect of vitamin B6 supplementation on colon gene expression in AOM-treated mice. We found that vitamin B6 supplementation downregulates Cd8a and Ccl8 mRNA expression, suggesting these candidate genes may play a protective role against colonic ACF development. Furthermore, we examined genomic affects of dietary vitamin B6, and showed that Reg3γ mRNA expression in colons is downregulated by vitamin B6. Conclusion This study provides an insight into the genomic activities of dietary vitamin B6 that may be protective against colon tumor development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text