Spinal Reflex Recovery after Dorsal Rhizotomy and Repair with Platelet-Rich Plasma (PRP) Gel Combined with Bioengineered Human Embryonic Stem Cells (hESCs)
| Autor: | Ângela Cristina Malheiros Luzo, Maria Helena Andrade Santana, Moníze Valéria Ramos da Silva, Alexandre Leite Rodrigues de Oliveira, Mateus Vidigal de Castro, Gabriela Bortolança Chiarotto, Sergiy Kyrylenko |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Article Subject business.industry medicine.medical_treatment Regeneration (biology) Rhizotomy Context (language use) Cell Biology Spinal cord RC31-1245 Embryonic stem cell Cell therapy medicine.anatomical_structure Platelet-rich plasma medicine excitatory synaptic circuits Axotomy dorsal root rhizotomy (DRZ) business Internal medicine Molecular Biology human embryonic stem cells (hESCs) Research Article |
| Zdroj: | Stem Cells International Stem Cells International, Vol 2020 (2020) |
| ISSN: | 1687-9678 1687-966X |
| DOI: | 10.1155/2020/8834360 |
| Popis: | Dorsal root rhizotomy (DRZ) is currently considered an untreatable injury, resulting in the loss of sensitive function and usually leading to neuropathic pain. In this context, we recently proposed a new surgical approach to treat DRZ that uses platelet-rich plasma (PRP) gel to restore the spinal reflex. Success was correlated with the reentry of primary afferents into the spinal cord. Here, aiming to enhance previous results, cell therapy with bioengineered human embryonic stem cells (hESCs) to overexpress fibroblast growth factor 2 (FGF2) was combined with PRP. For these experiments, adult female rats were submitted to a unilateral rhizotomy of the lumbar spinal dorsal roots, which was followed by root repair with PRP gel with or without bioengineered hESCs. One week after DRZ, the spinal cords were processed to evaluate changes in the glial response (GFAP and Iba-1) and excitatory synaptic circuits (VGLUT1) by immunofluorescence. Eight weeks postsurgery, the lumbar intumescences were processed for analysis of the repaired microenvironment by transmission electron microscopy. Spinal reflex recovery was evaluated by the electronic Von Frey method for eight weeks. The transcript levels for human FGF2 were over 37-fold higher in the induced hESCs than in the noninduced and the wildtype counterparts. Altogether, the results indicate that the combination of hESCs with PRP gel promoted substantial and prominent axonal regeneration processes after DRZ. Thus, the repair of dorsal roots, if done appropriately, may be considered an approach to regain sensory-motor function after dorsal root axotomy. |
| Databáze: | OpenAIRE |
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