Alternative Splicing Substantially Diversifies the Transcriptome during Early Photomorphogenesis and Correlates with the Energy Availability in Arabidopsis
| Autor: | Jonas Behr, Sascha Geue, Philipp Drewe-Boß, André Kahles, Fabian H. Sinz, Andreas Wachter, Gunnar Rätsch, Lisa Hartmann, Gabriele Wagner, Dominik M Obermüller, Theresa Wießner, Hsin-Chieh Lee |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Plant Science 01 natural sciences In Brief Transcriptome 03 medical and health sciences Splicing factor Gene Expression Regulation Plant Genetics Regulation of gene expression biology Arabidopsis Proteins Alternative splicing Plant physiology Cell Biology biology.organism_classification Alternative Splicing 030104 developmental biology Photomorphogenesis Signal transduction 010606 plant biology & botany Signal Transduction |
| Zdroj: | The Plant cell. 28(11) |
| ISSN: | 1532-298X |
| Popis: | Plants use light as source of energy and information to detect diurnal rhythms and seasonal changes. Sensing changing light conditions is critical to adjust plant metabolism and to initiate developmental transitions. Here, we analyzed transcriptome-wide alterations in gene expression and alternative splicing (AS) of etiolated seedlings undergoing photomorphogenesis upon exposure to blue, red, or white light. Our analysis revealed massive transcriptome reprogramming as reflected by differential expression of ∼20% of all genes and changes in several hundred AS events. For more than 60% of all regulated AS events, light promoted the production of a presumably protein-coding variant at the expense of an mRNA with nonsense-mediated decay-triggering features. Accordingly, AS of the putative splicing factor REDUCED RED-LIGHT RESPONSES IN CRY1CRY2 BACKGROUND1, previously identified as a red light signaling component, was shifted to the functional variant under light. Downstream analyses of candidate AS events pointed at a role of photoreceptor signaling only in monochromatic but not in white light. Furthermore, we demonstrated similar AS changes upon light exposure and exogenous sugar supply, with a critical involvement of kinase signaling. We propose that AS is an integration point of signaling pathways that sense and transmit information regarding the energy availability in plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|