Temperatures from 4 to 15 °C are suitable for preserving the fertilizing capacity of stallion semen stored for 22 h or more in INRA96 extender

Autor: Elisabeth Blesbois, Michèle Magistrini, Nicolas Levillain, Yoann Le Foll, J. M. Yvon, Marianne Vidament, G. Duchamp
Přispěvatelé: Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), IFCE, INRA, Vidament, Marianne, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), UE 1297 Unité Expérimentale de Physiologie Animale de l'Orfrasière, Institut National de la Recherche Agronomique (INRA)-Physiologie Animale et Systèmes d'Elevage (PHASE), Institut National de la Recherche Agronomique (INRA)-Unité Expérimentale de Physiologie Animale de l'Orfrasière (UE PAO), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2011
Předmět:
Male
sperme
medicine.medical_treatment
Acrosome reaction
reproduction animale
réaction acrosomique
Cryopreservation
law.invention
0302 clinical medicine
Cryoprotective Agents
Food Animals
law
Pregnancy
Small Animals
Sperm motility
Insemination
Artificial
Animal biology
030219 obstetrics & reproductive medicine
[SDV.BA]Life Sciences [q-bio]/Animal biology
Extender
Temperature
04 agricultural and veterinary sciences
Spermatozoa
horse
insémination animale
Fixed field
motility
spermatozoa
storage
fertility
acrosome reaction
Sperm Motility
Female
étalon
équin
medicine.medical_specialty
motilité
Semen
Biology
03 medical and health sciences
Animal science
Biologie animale
conservation du sperme
medicine
Animals
spermatozoïde
Horses
Gynecology
Equine
Artificial insemination
fertilité animale
0402 animal and dairy science
040201 dairy & animal science
Sperm
Fertility
Animal Science and Zoology
Semen Preservation
Zdroj: Theriogenology
Theriogenology, Elsevier, 2012, 78 (2), pp.297-307. ⟨10.1016/j.theriogenology.2012.01.018⟩
Theriogenology 2 (78), 297-307. (2012)
ISSN: 1879-3231
0093-691X
DOI: 10.1016/j.theriogenology.2012.01.018⟩
Popis: This study tested whether variable temperatures (from -0.5 to 15 degrees C) and air exposure could be used under laboratory and under field conditions to store stallion sperm diluted in extender INRA96 without loss of fertility. Experiment 1 (laboratory conditions) measured the effects of two 72 h storage conditions (5 degrees C with air vs. 15 degrees C without air). Experiment 2 (fixed field conditions) measured the effects of 22 h of storage without air in disposable containers maintained at four ambient temperatures (7 degrees C, 17 degrees C, 27 degrees C, 39 degrees C with semen at -0.5 degrees C to 3 degrees C, 4 degrees C to 7 degrees C, 8 degrees C to 10 degrees C, 12 degrees C to 15 degrees C, respectively). Per cycle pregnancy rate (PC) was measured after one artificial insemination (AI) in uterine body of 200 X 10(6) total spermatozoa, 7 h (Experiment 1) or 17 h (Experiment 2) before ovulation. In Experiment 1, PC was similar for both conditions (60% (n = 40 cycles) vs. 63% (n = 40), respectively, 5 stallions X 8 cycles). Only velocity VCL and ALH were slightly higher at 15 degrees C. In Experiment 2, PC was reduced when ambient temperature was low (semen at -0.5 degrees C to 3 degrees C; PC = 25%) rather than intermediate (semen at 4 degrees C to 7 degrees C; PC = 53%) or high (semen at 8 degrees C to 10 degrees C; PC = 50%) (4 stallions X 8 cycles) (P = 0.002). Sperm stored at -0.5 degrees C to 3 degrees C had lower acrosome integrity/responsiveness, similar membrane integrity (HOS test) and motilities, and higher VCL and ALH, than semen stored between 4 and 15 degrees C. These results demonstrate a wide tolerance of equine sperm to variable positive temperatures and air exposure for 22 h storage and more. However, temperatures close to 0 degrees C are detrimental for fertility.
Databáze: OpenAIRE
Externí odkaz: