Analysis of the Behavior of eryC Mutants of Brucella suis Attenuated in Macrophages
| Autor: | Sonja Burkhardt, Jean-Pierre Liautard, María Pilar Jiménez de Bagüés, Stephan Köhler |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Brucella suis
Immunology Mutant Virulence Erythritol Biology Microbiology Mice chemistry.chemical_compound Bacterial Proteins Animals Pathogen Mice Inbred BALB C Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Macrophages Wild type biology.organism_classification Complementation Infectious Diseases chemistry Mutation Parasitology Gene Deletion Spleen Sugar Alcohol Dehydrogenases Brucella melitensis |
| Zdroj: | Infection and Immunity. 73:6782-6790 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | The facultatively intracellular pathogen Brucella , characterized by its capacity to replicate in professional and non professional phagocytes, also causes abortion in ruminants. This property has been linked to the presence of erythritol in the placenta, as brucellae preferentially utilize erythritol. The ery operon encodes enzymes involved in erythritol metabolism, and a link with virulence has since been discussed. Allelic exchange mutants in eryC of Brucella suis were erythritol sensitive in vitro with a MIC of 1 to 5 mM of erythritol. Their multiplication in macrophage-like cells was 50- to 90-fold reduced, but complementation of the mutant restored wild-type levels of intracellular multiplication and the capacity to use erythritol as a sole carbon source. In vivo, the eryC mutant colonized the spleens of infected BALB/c mice to a significantly lower extent than the wild type and the complemented strain. Interestingly, eryC mutants that were in addition spontaneously erythritol tolerant nevertheless exhibited wild-type-like intramacrophagic and intramurine replication. We concluded from our results that erythritol was not an essential carbon source for the pathogen in the macrophage host cell but that the inactivation of the eryC gene significantly reduced the intramacrophagic and intramurine fitness of B. suis . |
| Databáze: | OpenAIRE |
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