Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells
| Autor: | Shujun Wang, Hua Yu, Xiaojun Ma, Guangwei Sun, Shijie Lv, Xin Guo, Yang Liu, Chang Liu, Feng Nan, Jianchun Lian, Xin He |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:Internal medicine Article Subject Oncogene Cell growth Mesenchymal stem cell Cell Biology Cell cycle Biology Umbilical cord Cell biology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Serial passage Immunology Wharton's jelly medicine Stem cell lcsh:RC31-1245 Molecular Biology Research Article |
| Zdroj: | Stem Cells International Stem Cells International, Vol 2016 (2016) |
| ISSN: | 1687-9678 1687-966X |
| DOI: | 10.1155/2016/9291013 |
| Popis: | Background and Objective. It is important to guarantee the quality of stem cells. Serial passage is the main approach to expand stem cells. This study evaluated effects of serial passage on the biological characteristics of human umbilical cord Wharton’s jelly-derived MSCs (WJ MSCs).Methods. Biological properties of WJ MSCs in the early (less than 10 passages, P10), middle (P11–20), and late (more than P20) phases including cell proliferation, cell cycle, phenotype, senescence, oncogene expression, stemness marker expression, and differentiation capacity were evaluated using flow cytometry, real-time PCR, immunocytofluorescence, and western blot.Results. It was found that there were no significant differences in cell proliferation, cell cycle, phenotype, and stemness marker expression in different phases. However, the expression of senescence-related gene, p21, and oncogene, c-Myc, was significantly upregulated in the late phase, which had close relations with the obviously increased cell senescence. Moreover, cardiac differentiation capability of WJ MSCs decreased whereas the propensity for neural differentiation increased significantly in the middle phase.Conclusions. This study reveals that WJ MSCs in the early and middle phases are relatively stable, and effect of serial passage on the lineage-specific differentiation should be considered carefully. |
| Databáze: | OpenAIRE |
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