| Autor: |
Liu, Lei, Zhou, Ying, Mao, Feng, Gu, Yujuan, Tang, Ziwei, Xin, Yi, Liu, Fuxia, Tang, Tang, Gao, Hui, Zhao, Xiangxiang |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.18230839 |
| Popis: |
Additional file 1: Fig. S1. GS3 is subject to alternative splicing. (A) GS3 AS variants were shown by agarose gel electrophoresis. (B) GS3 AS variants were sequenced by reverse primer. (C) GS3 AS variants from Huaidao 5 were sequenced by the forward primer. Fig. S2 Structures and sequences of GS3 alternative splicing variants. (A) Structures of GS3 alternative splicing variants. Gray boxes represent UTR. Black and red boxes indicate exons. Gray and blue lines denote introns. (B) Sequences of GS3 alternative splicing variants. Fig. S3. Clone number of GS3 alternative splicing variants. Fig. S4. Expression pattern analysis of (A) GS3.1 and (B) GS3.2 by qRT-PCR. R1-3, root in seedling, tillering and heading stage, respectively. St1-2, stem in elongation and heading stage, respectively. L1-3, leaf in seedling, tillering and heading stage, respectively. Sp, spikelet. P1-3, panicles with 2 mm, 3 cm and 5 cm length, respectively. En1-3, Endosperm of 3, 12 and 20 days after pollination, respectively. The results from three biological replicates are consistent. Data are shown as mean �� SEM from three technical replicates. Fig. S5. Expression analysis of GS3.1 and GS3.2m overexpressors. (A) Expression level analysis of GS3.1 and GS3.2m overexpressors by qRT-PCR. Ubiquitin was used as internal control. Data are shown as mean �� SEM. (B) Sequencing of the amplification products from GS3.2 and GS3.2.m The red box indicated the mutation between GS3.2 and GS3.2m. Table S1. Information of alternative splicing of GS3 homologs and effects on grain size. Table S2. Summary of grain traits of GS3 variants overexpressors. Table S3. List of primers used in this study. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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