Artificial spider silk supports and guides neurite extension in vitro
| Autor: | Juanita Francis, Magnus L. Hansson, Mattias K. Sköld, Jan Johansson, Tina Arndt, Christian Broman, Urmimala Chatterjee, Anna Rising |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cell Survival
Medical Materials Biomaterialvetenskap Silk Nerve guidance conduit Biocompatible Materials Biology Protein Engineering Biochemistry Mice Dorsal root ganglion Neurite extension Peripheral Nerve Injuries Peripheral nerve Cell Line Tumor Ganglia Spinal Neurites Genetics medicine Animals Humans recombinant spider silk Spider silk Vitronectin Autografts Molecular Biology Cell Proliferation Regeneration (biology) biomaterial Biomaterial Spiders nerve guidance conduit Recombinant Proteins In vitro Nerve Regeneration Rats Cell biology medicine.anatomical_structure peripheral nerve Biomaterials Science Laminin Biotechnology |
| Popis: | Surgical intervention with the use of autografts is considered the gold standard to treat peripheral nerve injuries. However, a biomaterial that supports and guides nerve growth would be an attractive alternative to overcome problems with limited availability, morbidity at the site of harvest, and nerve mismatches related to autografts. Native spider silk is a promising material for construction of nerve guidance conduit (NGC), as it enables regeneration of cm-long nerve injuries in sheep, but regulatory requirements for medical devices demand synthetic materials. Here, we use a recombinant spider silk protein (NT2RepCT) and a functionalized variant carrying a peptide derived from vitronectin (VN-NT2RepCT) as substrates for nerve growth support and neurite extension, using a dorsal root ganglion cell line, ND7/23. Two-dimensional coatings were benchmarked against poly-d-lysine and recombinant laminins. Both spider silk coatings performed as the control substrates with regards to proliferation, survival, and neurite growth. Furthermore, NT2RepCT and VN-NT2RepCT spun into continuous fibers in a biomimetic spinning set-up support cell survival, neurite growth, and guidance to an even larger extent than native spider silk. Thus, artificial spider silk is a promising biomaterial for development of NGCs. |
| Databáze: | OpenAIRE |
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