Potentiation of Inhaled Staphylococcal Enterotoxin B-Induced Toxicity by Lipopolysaccharide in Mice
| Autor: | Catherine L. Wilhelmsen, Gene O. Nelson, James E. Estep, Sina Bavari, Ross D. LeClaire, Robert E. Hunt |
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| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
Interleukin 2 Lipopolysaccharide 040301 veterinary sciences medicine.medical_treatment Biology Toxicology 030226 pharmacology & pharmacy Pathology and Forensic Medicine 0403 veterinary science Enterotoxins Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Receptor Molecular Biology Mice Inbred BALB C Dose-Response Relationship Drug Interleukin Drug Synergism 04 agricultural and veterinary sciences Cell Biology Pulmonary edema medicine.disease Macaca mulatta Cytokine chemistry Immunology Toxicity Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Toxicologic Pathology. 24:619-626 |
| ISSN: | 1533-1601 0192-6233 |
| DOI: | 10.1177/019262339602400513 |
| Popis: | Nonhuman primates are the established model for evaluating toxic responses to staphylococcal enterotoxins (SEs), as they react similarly to humans. Rodents are generally considered unresponsive to SEs. Binding affinities and T-cell reactivity suggest that SE binds more efficiently to primate major histocompatability complex class II receptors than to mouse receptors. We investigated the potentiation of staphylococcal enterotoxin B (SEB) inhalation toxicity by lipopolysaccharide (LPS) in BALB/c mice. Lethality occurred only when SEB was potentiated by LPS. Neither SEB nor LPS produced lethal effects alone. Temporal responses of interleukin 1α, tumor necrosis factor α, interleukin 2, and interferon-γ evoked by inhaled SEB were enhanced by LPS. By 24 hr after intoxication, serum cytokines decreased to baseline levels, and consistent pulmonary perivascular leukocytic infiltrates were evident histologically. Histologic lesions induced by inhalation exposure to SEB by mice, with or without potentiation by LPS, were similar to those in the rhesus monkey. Predominant pulmonary lesions included severe, diffuse interstitial and alveolar pulmonary edema, leukocytic infiltrates, mild perivascular edema, and alveolar fibrin deposition. Although the mechanism of aerosolized SEB-induced toxicity has not been completely resolved, similarities in histologic lesions, cytokine responses, and acute dose-response suggest the LPS-potentiated mouse model may be a credible alternative to the nonhuman primate model. |
| Databáze: | OpenAIRE |
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