Chemiluminescence Imaging of Superoxide Anion Detects Beta-Cell Function and Mass
| Autor: | Christopher H. Contag, Christian Stokes, Laura L. Bronsart |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell physiology Cellular respiration Pyridines Cell Respiration lcsh:Medicine Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Imaging Three-Dimensional In vivo Mice Inbred NOD Ranolazine Superoxides Coelenterazine Insulin-Secreting Cells Animals Humans lcsh:Science Multidisciplinary Chemistry Superoxide lcsh:R Imidazoles Organ Size In vitro 3. Good health Disease Models Animal 030104 developmental biology Glucose Biochemistry Hyperglycemia Pyrazines Luminescent Measurements Biophysics lcsh:Q 030217 neurology & neurosurgery Homeostasis Intracellular Research Article HeLa Cells |
| Zdroj: | PLoS ONE, Vol 11, Iss 1, p e0146601 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Superoxide anion is produced during normal cellular respiration and plays key roles in cellular physiology with its dysregulation being associated with a variety of diseases. Superoxide anion is a short-lived molecule and, therefore, its homeostatic regulation and role in biology and disease requires dynamic quantification with fine temporal resolution. Here we validated coelenterazine as a reporter of intracellular superoxide anion concentration and used it as a dynamic measure both in vitro and in vivo. Chemiluminescence was dependent upon superoxide anion levels, including those produced during cellular respiration, and concentrations varied both kinetically and temporally in response to physiologically relevant fluctuations in glucose levels. In vivo imaging with coelenterazine revealed that beta cells of the pancreas have increased levels of superoxide anion, which acted as a measure of beta-cell function and mass and could predict the susceptibility of mice to diabetes mellitus. Glucose response and regulation are key elements of cellular physiology and organismal biology, and superoxide anion appears to play a fundamental and dynamic role in both of these processes. |
| Databáze: | OpenAIRE |
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