Deposition of Host Matrix Proteins on Breast Implant Surfaces Facilitates Staphylococcus Epidermidis Biofilm Formation: In Vitro Analysis
| Autor: | Sarah Ortbal, Scott J. Hultgren, Terence M. Myckatyn, Jennifer N. Walker, Chloe L. Pinkner, Aaron J L Lynch, Jerome S. Pinkner |
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| Rok vydání: | 2019 |
| Předmět: |
SDRF
Breast Implants Virulence 030230 surgery Matrix (biology) Microbiology law.invention 03 medical and health sciences 0302 clinical medicine law Staphylococcus epidermidis Medicine Humans Breast Implantation biology business.industry Biofilm General Medicine biology.organism_classification In vitro Anti-Bacterial Agents Biofilms Breast implant Surgery business Type I collagen |
| Zdroj: | Aesthetic surgery journal. 40(3) |
| ISSN: | 1527-330X |
| Popis: | Background Staphylococcus epidermidis is a primary cause of breast implant-associated infection. S epidermidis possesses several virulence factors that enable it to bind both abiotic surfaces and host factors to form a biofilm. In addition S epidermidis colocalizes with matrix proteins coating explanted human breast implants. Objectives The authors sought to identify matrix proteins that S epidermidis may exploit to infect various breast implant surfaces in vitro. Methods A combination of in vitro assays was used to characterize S epidermidis strains isolated from human breast implants to gain a better understanding of how these bacteria colonize breast implant surfaces. These included determining the (1) minimum inhibitory and bactericidal concentrations for irrigation solutions commonly used to prevent breast implant contamination; (2) expression and carriage of polysaccharide intercellular adhesin and serine-aspartate repeat proteins, which bind fibrinogen (SdrG) and collagen (SdrF), respectively; and (3) biofilm formation on varying implant surface characteristics, in different growth media, and supplemented with fibrinogen and Types I and III collagen. Scanning electron microscopy and immunofluorescence staining analyses were performed to corroborate findings from these assays. Results Textured breast implant surfaces support greater bacterial biofilm formation at baseline, and the addition of collagen significantly increases biomass on all surfaces tested. We found that S epidermidis isolated from breast implants all encoded SdrF. Consistent with this finding, these strains had a clear affinity for Type I collagen, forming dense, highly structured biofilms in its presence. Conclusions The authors found that S epidermidis may utilize SdrF to interact with Type I collagen to form biofilm on breast implant surfaces. Level of Evidence: 5 |
| Databáze: | OpenAIRE |
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