Heterologous gene expression in an Escherichia coli population under starvation stress conditions
| Autor: | Renato Fani, Elena Mori, Romina Gallo, Elena Tamburini, Antonio Lazcano, Silvia Fancelli |
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| Rok vydání: | 1998 |
| Předmět: |
Transcription
Genetic Population Genetic Vectors Heterologous medicine.disease_cause Evolution Molecular chemistry.chemical_compound Plasmid RNA polymerase Genetics medicine Escherichia coli Histidine education Promoter Regions Genetic Molecular Biology Ecology Evolution Behavior and Systematics education.field_of_study biology Gene Transfer Techniques Promoter DNA-Directed RNA Polymerases Azospirillum brasilense biology.organism_classification Adaptation Physiological chemistry Biochemistry Heterologous expression Azospirillum Directed Molecular Evolution Plasmids |
| Zdroj: | Journal of molecular evolution. 47(3) |
| ISSN: | 0022-2844 |
| Popis: | A novel system to study the evolution of transcription signals in heterologous systems under selective starvation conditions is described. It is based on the plasmid-mediated transfer of his biosynthetic genes from Azospirillum brasilense into a heterologous Escherichia coli mutant population lacking histidine biosynthetic ability. We show that under highly selective stressful conditions, genetic changes in the donor plasmid lead to mutated sequences that are efficiently recognized as promoters by the E. coli RNA polymerase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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