Cyanine fluorophore derivatives with enhanced photostability
| Autor: | Daniel S. Terry, Yongfang Zhao, Peter Geggier, J. David Warren, Roger B. Altman, Zhou Zhou, Jonathan A. Javitch, Rachel A. Kolster, Scott C. Blanchard, Qinsi Zheng |
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| Rok vydání: | 2011 |
| Předmět: |
Fluorophore
CHO Cells Molecular Dynamics Simulation Photochemistry Biochemistry Fluorescence Article Cyclooctanes chemistry.chemical_compound Drug Stability Cricetinae Animals Humans Chromans Cyanine Molecular Biology Benzyl Alcohols Fluorescent Dyes Photobleaching Singlet Oxygen Receptors Dopamine D2 Singlet oxygen DNA Cell Biology Carbocyanines Kinetics chemistry Trolox Biotechnology |
| Zdroj: | Nature Methods. 9:68-71 |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/nmeth.1774 |
| Popis: | Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission. |
| Databáze: | OpenAIRE |
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