Study of Anti-Inflammatory and Analgesic Activity of Scorpion Toxins DKK-SP1/2 from Scorpion Buthus martensii Karsch (BmK)

Autor:	Chunli Li, Yan Li, Yongbo Song, Yunxia Liu, Yuchen Zhu, Mingze Gui, Junyu Ji, Liping Zhang
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine Buthus martensii Karsch (BmK) Health Toxicology and Mutagenesis Anti-Inflammatory Agents DKK-SP2 DKK-SP1 Pharmacology Toxicology law.invention Mice 0302 clinical medicine Plasmid law Cricetinae Buthus Analgesics biology Chemistry analgesia Recombinant Proteins Chromatography Gel Recombinant DNA Medicine medicine.symptom Plasmids medicine.drug_class Analgesic Scorpion Pain Scorpion Venoms Inflammation inflammatory Article Anti-inflammatory Scorpions 03 medical and health sciences Cricetulus biology.animal Escherichia coli medicine Animals Amino Acid Sequence Gene Library cDNA library biology.organism_classification Rats 030104 developmental biology 030217 neurology & neurosurgery
Zdroj:	Toxins Volume 13 Issue 7 Toxins, Vol 13, Iss 498, p 498 (2021)
ISSN:	2072-6651
DOI:	10.3390/toxins13070498
Popis:	Buthus martensii Karsch (BmK), is a kind of traditional Chinese medicine, which has been used for a long history for the treatment of many diseases, such as inflammation, pain and cancer. In this study, DKK-SP1/2/3 genes were screened and extracted from the cDNA library of BmK. The DKK-SP1/2/3 were expressed by using plasmid pSYPU-1b in E. coli BL21, and recombinant proteins were obtained by column chromatography. In the xylene-induced mouse ear swelling and carrageenan-induced rat paw swelling model, DKK-SP1 exerted a significant anti-inflammatory effect by inhibiting the expression of Nav1.8 channel. Meanwhile, the release of pro-inflammatory cytokines (COX-2, IL-6) was decreased significantly and the release of anti-inflammatory cytokines (IL-10) were elevated significantly. Moreover, DKK-SP1 could significantly decrease the Nav1.8 current in acutely isolated rat DRG neurons. In the acetic acid-writhing and ION-CCI model, DKK-SP2 displayed significant analgesic activity by inhibiting the expression of the Nav1.7 channel. Moreover, DKK-SP2 could significantly inhibit the Nav1.7 current in the hNav1.7-CHO cells.
Databáze:	OpenAIRE
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