Prevention by 1'-acetoxychavicol acetate of the induction but not growth of putative preneoplastic, glutathione S-transferase placental form-positive, focal lesions in the livers of rats fed a choline- deficient, L-amino acid-defined diet
| Autor: | Dai Nakae, Ayumi Denda, Koichi Koshimizu, Yoichi Konishi, Hajime Ohigashi, Hiroyuki Akai, Eijiro Okajima, Akira Murakami, Wakashi Kitayama, Hideki Kishida, Toshifumi Tsujiuchi, Yozo Kobayashi |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty DNA damage Placenta Endogeny Oxidative phosphorylation Choline chemistry.chemical_compound Liver Neoplasms Experimental Internal medicine medicine Animals Anticarcinogenic Agents Diethylnitrosamine Amino Acids Anticarcinogen Benzyl Alcohols Glutathione Transferase biology Terpenes General Medicine Glutathione Rats Inbred F344 Diet Rats medicine.anatomical_structure Glutathione S-transferase Endocrinology chemistry Hepatocyte Carcinogens biology.protein Precancerous Conditions |
| Zdroj: | Carcinogenesis. 19:1809-1814 |
| ISSN: | 1460-2180 |
| Popis: | The effects of 1'-acetoxychavicol acetate (ACA) on endogenous rat liver carcinogenesis because of chronic feeding of a choline-deficient, L-amino acid-defined (CDAA) diet were examined. Male Fischer 344 rats, 6 weeks old, received the CDAA diet containing ACA at doses of 0, 0.005, 0.010 and 0.050% for 12 weeks and were then killed. ACA decreased the numbers of putative preneoplastic, glutathione S-transferase placental form (GST-P)-positive, focal lesions developing in the livers of rats fed the CDAA diet but did not alter their sizes. At the same time, ACA reduced the levels of 8-hydroxyguanine, a parameter of oxidative DNA damage, but did not significantly affect generation of 2-thiobarbituric acid-reacting substances, indicators of oxidative extra-DNA damage, or hepatocyte proliferation. Furthermore, ACA did not exert any significant effects on the numbers or sizes of GST-P-positive lesions in the livers of rats when administered between weeks 2 and 8 after initiation with a single i.p. dose of 200 mg/kg body wt of N-nitrosodiethylamine. These results indicate that ACA prevents the CDAA diet-associated induction of putative preneoplastic lesions by reduction of oxidative DNA damage but does not affect their subsequent growth. |
| Databáze: | OpenAIRE |
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