Reconstitution in vitro of the components and conditions required for the oxidative cross-linking of extracellular proteins in French bean (Phaseolus vulgaris L.)
| Autor: | Przemyslaw Wojtaszek, Jonathan B. Trethowan, G. Paul Bolwell |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Hypersensitive response
(Hydroxy)proline-rich glycoprotein Biophysics Biochemistry Phaseolus vulgaris Defense response Cell wall Structural Biology Genetics Secretion Cysteine Molecular Biology Polyacrylamide gel electrophoresis Horseradish Peroxidase Glycoproteins Plant Proteins chemistry.chemical_classification Plants Medicinal biology food and beverages Fabaceae Hydrogen Peroxide Cell Biology Extracellular matrix Hydrogen-Ion Concentration biology.organism_classification Oxidative cross-linking In vitro reconstitution Cross-Linking Reagents chemistry biology.protein Phaseolus Glycoprotein Oxidation-Reduction Peroxidase |
| Zdroj: | FEBS Letters. (1):95-98 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(97)00166-X |
| Popis: | Oxidative cross linking of three (hydroxy)proline-rich cell wall proteins, known to be immobilised during the elicitor-induced oxidative burst in French bean cells, was modelled using peroxidases with cysteine or H2O2. Further reconstitution of the homologous system was achieved with a 46 kDa bean cell-wall peroxidase using conditions known to appertain in cell walls prior to the immobilisation. Thus, cell wall alkalinisation and secretion of a reductant have been reconstituted by addition of apoplastic fluid to the wall proteins and the 46 kDa peroxidase with resultant cross-linking. This is the first demonstration of the oxidative cross-linking of cell wall glycoproteins without the addition of external H2O2. © 1997 Federation of European Biochemical Societies. |
| Databáze: | OpenAIRE |
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