Construction and evaluation of a virG thyA double mutant of Shigella flexneri 2a as a candidate live-attenuated oral vaccine
| Autor: | Ayako Kohno, Hideo Nariuchi, Masanosuke Yoshikawa, N Okada, Hirofumi Danbara, Mitsuru Nakayama, Masao Takasaka, Yasuhiro Yoshikawa, Hiroyuki Shimada, C. Sasakawa, Masaki Toriumi |
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| Rok vydání: | 1995 |
| Předmět: |
Shigellosis
Genetic Vectors Guinea Pigs Keratoconjunctivitis Virulence Microbial Sensitivity Tests Vaccines Attenuated Shigella flexneri Microbiology Immune Tolerance medicine Animals Adverse effect Dysentery Bacillary General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Antibodies Bacterial Enterobacteriaceae Virology Vaccination Macaca fascicularis Infectious Diseases Immunization Genes Bacterial Bacterial Vaccines Mutation Molecular Medicine Female |
| Zdroj: | Vaccine. 13:1436-1440 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/0264-410x(95)00071-8 |
| Popis: | A virG thyA double mutant of Shigella flexneri 2a was constructed as a candidate live-attenuated oral vaccine. In the keratoconjunctivitis model it did not provoke any adverse reaction by itself on guinea pigs' eyes and completely protected them from provoking keratoconjunctivitis. When (2.7–4.8) × 1010 of the vaccine was inoculated intragastrically after 1 day fasting in cynomolgus monkeys three times at weekly intervals, a watery stool was observed at 40% as a side-effect. Upon intragastric challenge after 1 day fasting with 7.5 × 109 of the virulent strain four weeks after the last vaccination, a statistically significant difference was obtained in the mortality rate but not in the morbidity rate between the vaccine and the control group, although the clinical findings were less severe in the vaccine group than in the control group. These results together with the histopathological and immunological findings indicate that the vaccine deserve further detailed studies. |
| Databáze: | OpenAIRE |
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