Reference gene and protein expression levels in two different NAFLD mouse models
| Autor: | Layanne Cabral da Cunha Araujo, Silvana Bordin, Carla Roberta de Oliveira Carvalho |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Article Subject RC799-869 law.invention 03 medical and health sciences 0302 clinical medicine law Lipid droplet Internal medicine Reference genes Gene expression Nonalcoholic fatty liver disease medicine Reference gene Gene Polymerase chain reaction Glyceraldehyde 3-phosphate dehydrogenase Hepatology biology business.industry AMINOÁCIDOS Gastroenterology Diseases of the digestive system. Gastroenterology medicine.disease 030104 developmental biology Endocrinology biology.protein business 030217 neurology & neurosurgery Research Article |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Gastroenterology Research and Practice Gastroenterology Research and Practice, Vol 2020 (2020) |
| Popis: | The expression levels of some reference genes and proteins are used for data normalization and quantification. However, these levels can change in response to experimental conditions or treatments. Aim. The aim of this work was to evaluate reference gene and protein expression in models of nonalcoholic fatty liver disease, using mice fed with a high-fat diet (HFD) and mice that are genetically obese (ob/ob). Main Methods. Histological staining techniques were used to verify the morphology and quantify the amount of lipid droplets present in the liver. Real-time polymerase chain reaction and immunoblotting were employed for monitoring protein expression and gene expression levels, respectively. Key Finding. The results showed that there was a substantial increase in the amount of lipid droplets in the livers of HFD and ob/ob animals when compared to the standard diet (SD) group. There was an observed reduction in the expression of β-actin (10%), α-tubulin (6%), GAPDH (19%), and RPL3 (15%) genes when comparing the ob/ob group to the HFD group. Additionally, the ob/ob mice displayed GAPDH protein levels that were substantially, but not significantly, reduced when compared to SD. Significance. It was concluded that there are slight differences in the expression levels of reference genes and proteins in these two NAFLD animal models, and researchers should consider these alterations when working with these models. |
| Databáze: | OpenAIRE |
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